Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22400 | 3' | -55.7 | NC_005056.1 | + | 1147 | 0.67 | 0.462225 |
Target: 5'- uGCCUccauuUUCgggGCGCUGGCggUCAuuuuuuuUGCCGCa -3' miRNA: 3'- -CGGA-----AAG---CGUGACCGuaGGU-------GCGGCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 1313 | 0.66 | 0.549443 |
Target: 5'- gGUCgg-CGUgGCUGGCuUCCuCGCUGCg -3' miRNA: 3'- -CGGaaaGCG-UGACCGuAGGuGCGGCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 1352 | 0.67 | 0.473667 |
Target: 5'- cGCCgaccUCGC-CUgGGCGaCCAUGCaCGCc -3' miRNA: 3'- -CGGaa--AGCGuGA-CCGUaGGUGCG-GCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 2594 | 0.73 | 0.211905 |
Target: 5'- aGUCUUUCgaauaucaguuGCACUGGUAUC-GCGCCGg -3' miRNA: 3'- -CGGAAAG-----------CGUGACCGUAGgUGCGGCg -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 2971 | 0.72 | 0.242081 |
Target: 5'- cGCUgc-UGUACUGGCAgggguuuUCCGUGCCGCa -3' miRNA: 3'- -CGGaaaGCGUGACCGU-------AGGUGCGGCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 5701 | 0.66 | 0.527334 |
Target: 5'- cGCCgg-UGCGCUGGC--UCAC-CUGCa -3' miRNA: 3'- -CGGaaaGCGUGACCGuaGGUGcGGCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 5907 | 0.66 | 0.538349 |
Target: 5'- cGCCc--CGCGCcagGGCGg-CACGCCGg -3' miRNA: 3'- -CGGaaaGCGUGa--CCGUagGUGCGGCg -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 5949 | 0.67 | 0.494825 |
Target: 5'- aCCUgaCGCugUaccGGCGUCCACcGCC-Cg -3' miRNA: 3'- cGGAaaGCGugA---CCGUAGGUG-CGGcG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 6324 | 0.71 | 0.277203 |
Target: 5'- cGCCgugugUGCGaUGGCGaCCGCGUCGCu -3' miRNA: 3'- -CGGaaa--GCGUgACCGUaGGUGCGGCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 6426 | 1.15 | 0.000174 |
Target: 5'- cGCCUUUCGCACUGGCAUCCACGCCGCg -3' miRNA: 3'- -CGGAAAGCGUGACCGUAGGUGCGGCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 9570 | 0.67 | 0.484191 |
Target: 5'- aCCcaUCGCGCcgGGCGUUCGCGCa-- -3' miRNA: 3'- cGGaaAGCGUGa-CCGUAGGUGCGgcg -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 11882 | 0.69 | 0.384694 |
Target: 5'- uGCCgg-UGCaguaaaGCUGGCAgcggcaaCCGCGCCGg -3' miRNA: 3'- -CGGaaaGCG------UGACCGUa------GGUGCGGCg -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 12405 | 0.67 | 0.494825 |
Target: 5'- aCCUUUUGCggucaGCgaGGCAUCCgggugACGUCGUg -3' miRNA: 3'- cGGAAAGCG-----UGa-CCGUAGG-----UGCGGCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 12538 | 0.7 | 0.331856 |
Target: 5'- gGCCuUUUCGCGCgguggUGGCGUCCuguGCgGUa -3' miRNA: 3'- -CGG-AAAGCGUG-----ACCGUAGGug-CGgCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 13260 | 0.69 | 0.357591 |
Target: 5'- gGUCUUUUGUugUGGUAcUCugGCgGCu -3' miRNA: 3'- -CGGAAAGCGugACCGUaGGugCGgCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 16254 | 0.67 | 0.457073 |
Target: 5'- gGCUg-UUGCACUGGCAUCagucugucagaaGCUGCg -3' miRNA: 3'- -CGGaaAGCGUGACCGUAGgug---------CGGCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 18551 | 0.7 | 0.299701 |
Target: 5'- uGCCUguugCGCACUGacacgcgccagcGCGU-CACGCUGCc -3' miRNA: 3'- -CGGAaa--GCGUGAC------------CGUAgGUGCGGCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 19768 | 0.69 | 0.357591 |
Target: 5'- cGCCUgcugcCGCGaugaUGGCAUUgAUGCCGg -3' miRNA: 3'- -CGGAaa---GCGUg---ACCGUAGgUGCGGCg -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 21844 | 0.69 | 0.366475 |
Target: 5'- uGCCacgaaaGCGCaGGcCAUgCGCGCCGCu -3' miRNA: 3'- -CGGaaag--CGUGaCC-GUAgGUGCGGCG- -5' |
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22400 | 3' | -55.7 | NC_005056.1 | + | 23355 | 0.68 | 0.432781 |
Target: 5'- aGCuCUUUUGCucauccggcucACUGGUAUaucacCUAUGCCGCu -3' miRNA: 3'- -CG-GAAAGCG-----------UGACCGUA-----GGUGCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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