Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22401 | 3' | -55.4 | NC_005056.1 | + | 5855 | 0.66 | 0.530268 |
Target: 5'- -aCACCG-GCAaaACGUGgaCGCCCGAa-- -3' miRNA: 3'- ugGUGGCuCGU--UGUAC--GCGGGCUauu -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 5698 | 0.66 | 0.501432 |
Target: 5'- uGCCGCCGGugcgcuggcucaccuGCAACGUGCgacacaGCUCGAc-- -3' miRNA: 3'- -UGGUGGCU---------------CGUUGUACG------CGGGCUauu -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 18459 | 0.66 | 0.486192 |
Target: 5'- aACCACCGcgcagcuuaaccGGCAGCGUGaCGCgCUGGc-- -3' miRNA: 3'- -UGGUGGC------------UCGUUGUAC-GCG-GGCUauu -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 6517 | 0.66 | 0.47224 |
Target: 5'- cGCUACaGGGCGACAcgcucgacgccauuUGUGUCCGGUAu -3' miRNA: 3'- -UGGUGgCUCGUUGU--------------ACGCGGGCUAUu -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 26478 | 0.68 | 0.413629 |
Target: 5'- -gCACCGGGCuuuuauUGCGCCgGAUGu -3' miRNA: 3'- ugGUGGCUCGuugu--ACGCGGgCUAUu -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 8842 | 0.68 | 0.394149 |
Target: 5'- cGCCGCCgGAGCcgguCAccCGCCCGGUGGa -3' miRNA: 3'- -UGGUGG-CUCGuu--GUacGCGGGCUAUU- -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 24657 | 0.68 | 0.366068 |
Target: 5'- aACaGCC-AGCAGCuAUGCGCCgGAUAAu -3' miRNA: 3'- -UGgUGGcUCGUUG-UACGCGGgCUAUU- -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 9341 | 0.68 | 0.366068 |
Target: 5'- cACCgguaaaGCCGAGCAGaggacGCGCCgGAUAAu -3' miRNA: 3'- -UGG------UGGCUCGUUgua--CGCGGgCUAUU- -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 9519 | 0.7 | 0.314163 |
Target: 5'- cGCUGCCGugugcagaccGGCGGCAUGUGCaCCGAc-- -3' miRNA: 3'- -UGGUGGC----------UCGUUGUACGCG-GGCUauu -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 1771 | 0.71 | 0.247079 |
Target: 5'- -aCACCGuGGCGACA-GCGCCgGAUGc -3' miRNA: 3'- ugGUGGC-UCGUUGUaCGCGGgCUAUu -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 8832 | 0.71 | 0.240405 |
Target: 5'- cUCACUGAcCAGCGUGCGCUCGGUc- -3' miRNA: 3'- uGGUGGCUcGUUGUACGCGGGCUAuu -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 3977 | 0.76 | 0.113919 |
Target: 5'- aGCCACCGgcGGUGGCGUGaCGCCCuGAUGAc -3' miRNA: 3'- -UGGUGGC--UCGUUGUAC-GCGGG-CUAUU- -5' |
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22401 | 3' | -55.4 | NC_005056.1 | + | 6871 | 1.06 | 0.000659 |
Target: 5'- aACCACCGAGCAACAUGCGCCCGAUAAa -3' miRNA: 3'- -UGGUGGCUCGUUGUACGCGGGCUAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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