Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22402 | 3' | -52.6 | NC_005056.1 | + | 9659 | 0.65 | 0.745477 |
Target: 5'- gCACCUGuucccccaCGGaCGGUGCCCAccauguGCg -3' miRNA: 3'- aGUGGACuauug---GCC-GCUAUGGGU------CG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 18906 | 0.65 | 0.745477 |
Target: 5'- uUCGCCaGugccauauucagcGUGACCGGCGuUGCCgccugaauagccgCAGCa -3' miRNA: 3'- -AGUGGaC-------------UAUUGGCCGCuAUGG-------------GUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 2128 | 0.66 | 0.736635 |
Target: 5'- ---aCUGuuUAACCGGgGAcGCgCCAGCg -3' miRNA: 3'- agugGACu-AUUGGCCgCUaUG-GGUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 10249 | 0.66 | 0.736635 |
Target: 5'- gUCGCCUGuu-ACaUGGCGGUgcugaaaugggaACCCcGCg -3' miRNA: 3'- -AGUGGACuauUG-GCCGCUA------------UGGGuCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 4167 | 0.66 | 0.736635 |
Target: 5'- uUCACCguugAACCGuCGGUcACCCAGa -3' miRNA: 3'- -AGUGGacuaUUGGCcGCUA-UGGGUCg -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 8080 | 0.66 | 0.725479 |
Target: 5'- gUCugCUGuucACUGGCGAcguuguucacccUGUCCAGCg -3' miRNA: 3'- -AGugGACuauUGGCCGCU------------AUGGGUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 9670 | 0.66 | 0.714219 |
Target: 5'- -uGCCgggGAUuuauuCCGGUGAUaACCCcGCg -3' miRNA: 3'- agUGGa--CUAuu---GGCCGCUA-UGGGuCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 20342 | 0.67 | 0.679952 |
Target: 5'- -aGCCUGA---CCGGUGGUACUgcgccggggacaCAGCu -3' miRNA: 3'- agUGGACUauuGGCCGCUAUGG------------GUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 4312 | 0.67 | 0.668411 |
Target: 5'- cCACCgacaccGCCGGUGGcACCgAGCg -3' miRNA: 3'- aGUGGacuau-UGGCCGCUaUGGgUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 7603 | 0.67 | 0.649871 |
Target: 5'- gCACCUGGgcgauuCUGGCGAcgguuaucuguguggUugCCGGUg -3' miRNA: 3'- aGUGGACUauu---GGCCGCU---------------AugGGUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 18331 | 0.67 | 0.645227 |
Target: 5'- uUCGCCUGAgcACgGGUcgGAcGCUCGGUg -3' miRNA: 3'- -AGUGGACUauUGgCCG--CUaUGGGUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 19759 | 0.67 | 0.633609 |
Target: 5'- cCGCgaUGAUGgcauugauGCCGGUGAUAaCCGGCc -3' miRNA: 3'- aGUGg-ACUAU--------UGGCCGCUAUgGGUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 18620 | 0.67 | 0.633609 |
Target: 5'- -----cGAUGGCCGGUguuaaguugcuGAUGCCCGGUu -3' miRNA: 3'- aguggaCUAUUGGCCG-----------CUAUGGGUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 1684 | 0.69 | 0.552854 |
Target: 5'- cCACCggccaccACCGGCGGUGCC-AGUa -3' miRNA: 3'- aGUGGacuau--UGGCCGCUAUGGgUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 20429 | 0.69 | 0.552854 |
Target: 5'- gCAuCCUG-UAACUGGCGGUcaagcugugucCCCGGCg -3' miRNA: 3'- aGU-GGACuAUUGGCCGCUAu----------GGGUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 5575 | 0.69 | 0.538137 |
Target: 5'- cCACCUccucGGUGAacauguaCGGCGGggugcggcgguguuUACCCGGCa -3' miRNA: 3'- aGUGGA----CUAUUg------GCCGCU--------------AUGGGUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 10204 | 0.69 | 0.519101 |
Target: 5'- aUCACCcgUGAgccGACCGGCG-UGCgCAGg -3' miRNA: 3'- -AGUGG--ACUa--UUGGCCGCuAUGgGUCg -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 4882 | 0.7 | 0.493785 |
Target: 5'- gCAUC-GGUAaccuGCCGGCGGUAcgcgucccguacuuCCCGGCg -3' miRNA: 3'- aGUGGaCUAU----UGGCCGCUAU--------------GGGUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 5422 | 0.7 | 0.486191 |
Target: 5'- gCGUCUGGauACCGGCGAUAUugCCGGUg -3' miRNA: 3'- aGUGGACUauUGGCCGCUAUG--GGUCG- -5' |
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22402 | 3' | -52.6 | NC_005056.1 | + | 22011 | 0.7 | 0.475442 |
Target: 5'- -aGCCUGAUacuuuGACCGGCGuuuCCguGUa -3' miRNA: 3'- agUGGACUA-----UUGGCCGCuauGGguCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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