Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22404 | 5' | -52 | NC_005056.1 | + | 16081 | 0.73 | 0.320801 |
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Target: 5'- gACGGCACCGGCgaaGaCGAAGA-AACGAa -3' miRNA: 3'- -UGCCGUGGCCGa--C-GUUUUUgUUGCUg -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 20287 | 0.74 | 0.296246 |
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Target: 5'- cACGGCACCGGCUggccGCucuuAUGugGACc -3' miRNA: 3'- -UGCCGUGGCCGA----CGuuuuUGUugCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 12844 | 0.74 | 0.280703 |
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Target: 5'- gACGGCGCUGGCgggGCuugcuACuGCGGCa -3' miRNA: 3'- -UGCCGUGGCCGa--CGuuuu-UGuUGCUG- -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 19033 | 0.78 | 0.150073 |
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Target: 5'- uCGGC-CCGGUUGCGAAGACGGCa-- -3' miRNA: 3'- uGCCGuGGCCGACGUUUUUGUUGcug -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 4577 | 0.79 | 0.141436 |
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Target: 5'- gGCGGUcgGCUGGCUGCAGAAauaccGCAAUGAa -3' miRNA: 3'- -UGCCG--UGGCCGACGUUUU-----UGUUGCUg -5' |
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| 22404 | 5' | -52 | NC_005056.1 | + | 7790 | 1.1 | 0.000764 |
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Target: 5'- cACGGCACCGGCUGCAAAAACAACGACg -3' miRNA: 3'- -UGCCGUGGCCGACGUUUUUGUUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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