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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22405 | 5' | -54.6 | NC_005056.1 | + | 26482 | 0.66 | 0.585166 |
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Target: 5'- cCGuGCAccGGGCuuuuauuGCGCCGGAUGuaGUCAu -3' miRNA: 3'- -GCuCGUcuCCUG-------CGCGGCCUAU--UAGU- -5' |
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| 22405 | 5' | -54.6 | NC_005056.1 | + | 6973 | 0.67 | 0.541161 |
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Target: 5'- aGGGCAGGGGGaaaaGUaauGCCGG-UAAUUAa -3' miRNA: 3'- gCUCGUCUCCUg---CG---CGGCCuAUUAGU- -5' |
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| 22405 | 5' | -54.6 | NC_005056.1 | + | 24663 | 0.68 | 0.486504 |
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Target: 5'- -cAGCAGcu-AUGCGCCGGAUAAUUg -3' miRNA: 3'- gcUCGUCuccUGCGCGGCCUAUUAGu -5' |
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| 22405 | 5' | -54.6 | NC_005056.1 | + | 17781 | 0.7 | 0.349884 |
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Target: 5'- cCGAGCAgGAGGucgcCGCGCUGGAa----- -3' miRNA: 3'- -GCUCGU-CUCCu---GCGCGGCCUauuagu -5' |
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| 22405 | 5' | -54.6 | NC_005056.1 | + | 21229 | 0.71 | 0.308159 |
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Target: 5'- uGAaCAccGGGGCGCGCCGGAUGugGUCAc -3' miRNA: 3'- gCUcGUc-UCCUGCGCGGCCUAU--UAGU- -5' |
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| 22405 | 5' | -54.6 | NC_005056.1 | + | 9330 | 1.09 | 0.000577 |
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Target: 5'- cCGAGCAGAGGACGCGCCGGAUAAUCAa -3' miRNA: 3'- -GCUCGUCUCCUGCGCGGCCUAUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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