Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22407 | 3' | -54.5 | NC_005056.1 | + | 9790 | 1.11 | 0.00043 |
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Target: 5'- aCUCAAUCACCGCCCCGUCAGGGAAACg -3' miRNA: 3'- -GAGUUAGUGGCGGGGCAGUCCCUUUG- -5' |
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| 22407 | 3' | -54.5 | NC_005056.1 | + | 8951 | 0.71 | 0.310021 |
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Target: 5'- uUgAAUCGCUGUCaCCGUCAGGGcgucGGCg -3' miRNA: 3'- gAgUUAGUGGCGG-GGCAGUCCCu---UUG- -5' |
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| 22407 | 3' | -54.5 | NC_005056.1 | + | 8518 | 0.68 | 0.478899 |
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Target: 5'- aUCAGUCAgCGCCCUgcgcaggcuGUCGGGuuuGAGCa -3' miRNA: 3'- gAGUUAGUgGCGGGG---------CAGUCCc--UUUG- -5' |
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| 22407 | 3' | -54.5 | NC_005056.1 | + | 19154 | 0.68 | 0.489585 |
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Target: 5'- -cCGAUUACagGCUCCGaCAGGGAAAg -3' miRNA: 3'- gaGUUAGUGg-CGGGGCaGUCCCUUUg -5' |
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| 22407 | 3' | -54.5 | NC_005056.1 | + | 6122 | 0.66 | 0.578466 |
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Target: 5'- uUUAAUCACCGgCUCGUCGccugccgguugcGGGAuGACc -3' miRNA: 3'- gAGUUAGUGGCgGGGCAGU------------CCCU-UUG- -5' |
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| 22407 | 3' | -54.5 | NC_005056.1 | + | 9019 | 0.66 | 0.624332 |
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Target: 5'- aCUCAGUCGCCgacGCCCUGaCGGuGAcAGCg -3' miRNA: 3'- -GAGUUAGUGG---CGGGGCaGUCcCU-UUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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