Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22407 | 5' | -61.5 | NC_005056.1 | + | 20483 | 0.66 | 0.318364 |
Target: 5'- -uGCAUCaUGCUGGCaCGGGCGcGCGCa -3' miRNA: 3'- ccCGUAG-GCGGCUGcGUCUGC-CGCGc -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 9515 | 0.66 | 0.310723 |
Target: 5'- cGGUcgCUGCCGuguGCAGAcCGGCgGCa -3' miRNA: 3'- cCCGuaGGCGGCug-CGUCU-GCCG-CGc -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 1288 | 0.66 | 0.303223 |
Target: 5'- aGGGCGUgcauggUCGCCcaGGCGaGGuCGGCGUGg -3' miRNA: 3'- -CCCGUA------GGCGG--CUGCgUCuGCCGCGC- -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 2031 | 0.66 | 0.303223 |
Target: 5'- aGGGCGUUaugacugaCGUCGAuuuccaCGCGuucGGCGGCGCu -3' miRNA: 3'- -CCCGUAG--------GCGGCU------GCGU---CUGCCGCGc -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 6414 | 0.66 | 0.300261 |
Target: 5'- uGGCAUCCacgccgcgguaacGCucauaaagCGACGCGGucgccaucgcacacACGGCGCGc -3' miRNA: 3'- cCCGUAGG-------------CG--------GCUGCGUC--------------UGCCGCGC- -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 8092 | 0.66 | 0.292957 |
Target: 5'- aGGGCAgacaaaaacgaGCUGGCGCAGGUGGCacuGCGu -3' miRNA: 3'- -CCCGUagg--------CGGCUGCGUCUGCCG---CGC- -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 1764 | 0.67 | 0.261142 |
Target: 5'- cGGGCGggaGCCGAacggauugGCGGcaaACGGCGCa -3' miRNA: 3'- -CCCGUaggCGGCUg-------CGUC---UGCCGCGc -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 21127 | 0.67 | 0.253964 |
Target: 5'- cGGGCAggucgggCUGCCGguucgugGCGCuguccuGACGGUGUa -3' miRNA: 3'- -CCCGUa------GGCGGC-------UGCGu-----CUGCCGCGc -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 9584 | 0.67 | 0.235815 |
Target: 5'- cGGuGCAcaugCCGCCGGucUGCAcACGGCaGCGa -3' miRNA: 3'- -CC-CGUa---GGCGGCU--GCGUcUGCCG-CGC- -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 7750 | 0.68 | 0.223942 |
Target: 5'- cGGUG-CCG-UGGCGCAGACGG-GCGg -3' miRNA: 3'- cCCGUaGGCgGCUGCGUCUGCCgCGC- -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 18584 | 0.68 | 0.223942 |
Target: 5'- uGGGCG-CUGCCGGuguggggauUGCGG-CGGCGgGa -3' miRNA: 3'- -CCCGUaGGCGGCU---------GCGUCuGCCGCgC- -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 154 | 0.68 | 0.223362 |
Target: 5'- gGGGUGUaaaaaagCCGCC--CGCAGGCGGCGa- -3' miRNA: 3'- -CCCGUA-------GGCGGcuGCGUCUGCCGCgc -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 18698 | 0.68 | 0.212026 |
Target: 5'- uGGCAaaagacUCCGCCGAaauggccgcacucCGCAaGCaGGCGCGc -3' miRNA: 3'- cCCGU------AGGCGGCU-------------GCGUcUG-CCGCGC- -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 11433 | 0.68 | 0.201719 |
Target: 5'- cGGCAaaucugCUGCCGugGCuggcGugGGCGUu -3' miRNA: 3'- cCCGUa-----GGCGGCugCGu---CugCCGCGc -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 5624 | 0.69 | 0.181437 |
Target: 5'- cGGGUAUCCacagacucaCCGGCaGC-GugGGCGCGc -3' miRNA: 3'- -CCCGUAGGc--------GGCUG-CGuCugCCGCGC- -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 4034 | 0.69 | 0.171988 |
Target: 5'- aGGGCGUcaccgccaCCGCCgGugGCuuugcuGCGGCGCu -3' miRNA: 3'- -CCCGUA--------GGCGG-CugCGuc----UGCCGCGc -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 18818 | 0.7 | 0.16924 |
Target: 5'- cGGCugcaucAUCCGCCGAgGCGGcaguauugucaccgaGCuGGCGCGc -3' miRNA: 3'- cCCG------UAGGCGGCUgCGUC---------------UG-CCGCGC- -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 9600 | 0.7 | 0.162982 |
Target: 5'- uGGGCA-CCGuCCGugGgGGAacaGGUGCu -3' miRNA: 3'- -CCCGUaGGC-GGCugCgUCUg--CCGCGc -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 5962 | 0.71 | 0.13846 |
Target: 5'- cGGCGUgCCGCCcuGGCGCGG--GGCGCa -3' miRNA: 3'- cCCGUA-GGCGG--CUGCGUCugCCGCGc -5' |
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22407 | 5' | -61.5 | NC_005056.1 | + | 5670 | 0.71 | 0.13846 |
Target: 5'- aGGCAugUCUGCCGugGCGGuCaGUGCa -3' miRNA: 3'- cCCGU--AGGCGGCugCGUCuGcCGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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