Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22409 | 3' | -53 | NC_005056.1 | + | 19130 | 0.66 | 0.704901 |
Target: 5'- uACGGGUAGcagUGCCGuuGUCA-CCCg -3' miRNA: 3'- -UGUCCGUUaa-AUGGUggCGGUaGGGg -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 7686 | 0.66 | 0.681923 |
Target: 5'- uACGGGCGGga-GCUGCCGCCugaAUCgCUu -3' miRNA: 3'- -UGUCCGUUaaaUGGUGGCGG---UAGgGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 17206 | 0.66 | 0.681923 |
Target: 5'- -gAGGUcuaccGGUgcGCUGCCGuUCAUCCCCg -3' miRNA: 3'- ugUCCG-----UUAaaUGGUGGC-GGUAGGGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 17970 | 0.66 | 0.681923 |
Target: 5'- gGCGGcCAGUgaaaUAUCACUGCCAcaUCCgCCa -3' miRNA: 3'- -UGUCcGUUAa---AUGGUGGCGGU--AGG-GG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 6519 | 0.66 | 0.669188 |
Target: 5'- uGCAGcGCGGcUUguccuggauacgcGCCACCGCCcagcgcauAUCCCg -3' miRNA: 3'- -UGUC-CGUUaAA-------------UGGUGGCGG--------UAGGGg -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 10635 | 0.66 | 0.658732 |
Target: 5'- cACGGGCGGUgaugcugGCCugUGCCG-CCg- -3' miRNA: 3'- -UGUCCGUUAaa-----UGGugGCGGUaGGgg -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 10690 | 0.66 | 0.658732 |
Target: 5'- cACAGGCcagc-AUCACCGCCcgugcagCCUCa -3' miRNA: 3'- -UGUCCGuuaaaUGGUGGCGGua-----GGGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 30660 | 0.66 | 0.647088 |
Target: 5'- cCAGGCAAUUaugucaagGCCuCCGUCAccacuauuUCCUCg -3' miRNA: 3'- uGUCCGUUAAa-------UGGuGGCGGU--------AGGGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 208 | 0.66 | 0.647088 |
Target: 5'- uGCGGGCGGcuuuUUUACaCcCCGUCAUCacaCCCu -3' miRNA: 3'- -UGUCCGUU----AAAUG-GuGGCGGUAG---GGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 11060 | 0.66 | 0.647088 |
Target: 5'- cACGGucacGCGGUcgGCCACCGgCCugacGUCCUCa -3' miRNA: 3'- -UGUC----CGUUAaaUGGUGGC-GG----UAGGGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 9741 | 0.67 | 0.600479 |
Target: 5'- cGCAgacGGCGGgggUUAUCACCGgaauaaAUCCCCg -3' miRNA: 3'- -UGU---CCGUUa--AAUGGUGGCgg----UAGGGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 1219 | 0.68 | 0.565799 |
Target: 5'- --cGGCAAaaaaaAUgACCGCCAgcgCCCCg -3' miRNA: 3'- uguCCGUUaaa--UGgUGGCGGUa--GGGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 10078 | 0.69 | 0.487376 |
Target: 5'- --cGGUug--UGCCGCCGCUGUCgCCg -3' miRNA: 3'- uguCCGuuaaAUGGUGGCGGUAGgGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 15747 | 0.7 | 0.465892 |
Target: 5'- gGCAGGCAAUUcGC--CCGCCuGUCCUg -3' miRNA: 3'- -UGUCCGUUAAaUGguGGCGG-UAGGGg -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 5495 | 0.7 | 0.424469 |
Target: 5'- cCGGGUAAac-ACCGCCGCa--CCCCg -3' miRNA: 3'- uGUCCGUUaaaUGGUGGCGguaGGGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 18663 | 0.71 | 0.394886 |
Target: 5'- cACcGGCcaucgUUcCCGCCGCCGcaaUCCCCa -3' miRNA: 3'- -UGuCCGuua--AAuGGUGGCGGU---AGGGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 26105 | 0.71 | 0.366669 |
Target: 5'- uCGGGUAAUaaccaACCACCGCCAgccaUUCCUg -3' miRNA: 3'- uGUCCGUUAaa---UGGUGGCGGU----AGGGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 21593 | 0.73 | 0.282988 |
Target: 5'- uGCGGGauuuuUUUACCACUgGCCGUCaCCCc -3' miRNA: 3'- -UGUCCguu--AAAUGGUGG-CGGUAG-GGG- -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 26813 | 0.73 | 0.275506 |
Target: 5'- gGCAGGCAuauucUGCCAUUGCCcgCCUg -3' miRNA: 3'- -UGUCCGUuaa--AUGGUGGCGGuaGGGg -5' |
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22409 | 3' | -53 | NC_005056.1 | + | 28901 | 0.74 | 0.268183 |
Target: 5'- --uGGCAAccaUUGCC-CCGCCAgacaUCCCCg -3' miRNA: 3'- uguCCGUUa--AAUGGuGGCGGU----AGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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