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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22410 | 5' | -54.6 | NC_005056.1 | + | 2060 | 0.67 | 0.517989 |
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Target: 5'- cCGGAggUCUucUCUGUgcggacgGCCAGUGGCGg -3' miRNA: 3'- uGCCUuuGGAc-AGACG-------CGGUCAUCGU- -5' |
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| 22410 | 5' | -54.6 | NC_005056.1 | + | 7622 | 0.68 | 0.423589 |
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Target: 5'- gACGGuuAUCUGUgUGguUGCCGGUGGUg -3' miRNA: 3'- -UGCCuuUGGACAgAC--GCGGUCAUCGu -5' |
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| 22410 | 5' | -54.6 | NC_005056.1 | + | 8753 | 0.67 | 0.506926 |
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Target: 5'- aACGuGAAGCCcuuuuucUGgCUGCGUCGGUGGUc -3' miRNA: 3'- -UGC-CUUUGG-------ACaGACGCGGUCAUCGu -5' |
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| 22410 | 5' | -54.6 | NC_005056.1 | + | 11775 | 0.68 | 0.433691 |
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Target: 5'- cCGGcuACCUGUU--CGCCGGUGGUg -3' miRNA: 3'- uGCCuuUGGACAGacGCGGUCAUCGu -5' |
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| 22410 | 5' | -54.6 | NC_005056.1 | + | 12919 | 0.9 | 0.013584 |
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Target: 5'- uACGGAAACCUGUCUGC-CgCAGUAGCAa -3' miRNA: 3'- -UGCCUUUGGACAGACGcG-GUCAUCGU- -5' |
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| 22410 | 5' | -54.6 | NC_005056.1 | + | 19859 | 0.69 | 0.384635 |
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Target: 5'- cUGGGAGCCUGUCaGCGCauucuuUGGCGg -3' miRNA: 3'- uGCCUUUGGACAGaCGCGguc---AUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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