Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22413 | 5' | -57.3 | NC_005056.1 | + | 9595 | 0.66 | 0.440044 |
Target: 5'- -cUGAaGCCAGUCGGUgcacaugccGCCGGUcUGCa -3' miRNA: 3'- cuACUgCGGUCAGUCG---------CGGUCAcGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 8317 | 0.66 | 0.430077 |
Target: 5'- -cUGGCGUCuGUgCAG-GCCGG-GCGCu -3' miRNA: 3'- cuACUGCGGuCA-GUCgCGGUCaCGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 19601 | 0.66 | 0.430077 |
Target: 5'- -uUGugGCC-GUCuGUGUgagcuuacgCAGUGCGCc -3' miRNA: 3'- cuACugCGGuCAGuCGCG---------GUCACGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 1608 | 0.67 | 0.373194 |
Target: 5'- -uUGAUGCCAccggccucgguGUCGGCGuguuCCAgcucGUGCGCu -3' miRNA: 3'- cuACUGCGGU-----------CAGUCGC----GGU----CACGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 11376 | 0.67 | 0.373194 |
Target: 5'- uGAUGA-GCCGGUCGGCaG-CAGU-CGCg -3' miRNA: 3'- -CUACUgCGGUCAGUCG-CgGUCAcGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 16640 | 0.68 | 0.319208 |
Target: 5'- uGUGACGCCugacucguuaagcaGGUCAGCaUCGGUGC-Cg -3' miRNA: 3'- cUACUGCGG--------------UCAGUCGcGGUCACGcG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 9510 | 0.68 | 0.305681 |
Target: 5'- ---aACGCCGGUCGcUGCC-GUGUGCa -3' miRNA: 3'- cuacUGCGGUCAGUcGCGGuCACGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 5418 | 0.69 | 0.282893 |
Target: 5'- uGUGGCGUCuGgauacCGGCgauauuGCCGGUGCGCu -3' miRNA: 3'- cUACUGCGGuCa----GUCG------CGGUCACGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 12797 | 0.69 | 0.275601 |
Target: 5'- --cGcCGCCAGcucguUCAGCGUauuCAGUGCGUc -3' miRNA: 3'- cuaCuGCGGUC-----AGUCGCG---GUCACGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 18484 | 0.71 | 0.222606 |
Target: 5'- cGUGACGCgCuGgCGcGUGUCAGUGCGCa -3' miRNA: 3'- cUACUGCG-GuCaGU-CGCGGUCACGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 4060 | 0.71 | 0.210211 |
Target: 5'- -cUGACGCCGGUCAGCaGUucgucauCAGgGCGUc -3' miRNA: 3'- cuACUGCGGUCAGUCG-CG-------GUCaCGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 6001 | 0.74 | 0.130643 |
Target: 5'- --gGACGCCGGUaCAGCGUCAG-GUGa -3' miRNA: 3'- cuaCUGCGGUCA-GUCGCGGUCaCGCg -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 8527 | 0.78 | 0.064521 |
Target: 5'- cGUGACGgcaUCAGUCAGCGCCc-UGCGCa -3' miRNA: 3'- cUACUGC---GGUCAGUCGCGGucACGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 5684 | 0.8 | 0.04779 |
Target: 5'- cGUGACGCUGGUgAuGcCGCCGGUGCGCu -3' miRNA: 3'- cUACUGCGGUCAgU-C-GCGGUCACGCG- -5' |
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22413 | 5' | -57.3 | NC_005056.1 | + | 19703 | 1.1 | 0.000213 |
Target: 5'- cGAUGACGCCAGUCAGCGCCAGUGCGCc -3' miRNA: 3'- -CUACUGCGGUCAGUCGCGGUCACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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