Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22414 | 5' | -53.8 | NC_005056.1 | + | 9855 | 0.66 | 0.626989 |
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Target: 5'- aCCgGAagcugucacgcUGGCCGUuuuaauuccgcuugcCGUGAguGCACUGg -3' miRNA: 3'- gGGgCU-----------ACCGGCA---------------GUAUUguCGUGAC- -5' |
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| 22414 | 5' | -53.8 | NC_005056.1 | + | 19149 | 0.67 | 0.599381 |
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Target: 5'- cCCCUGAUGuuaUUGUCAU-GCAGCACa- -3' miRNA: 3'- -GGGGCUACc--GGCAGUAuUGUCGUGac -5' |
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| 22414 | 5' | -53.8 | NC_005056.1 | + | 17900 | 0.67 | 0.587922 |
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Target: 5'- aCCUGAUGGCgGaUGUGGCAGUgauauuucACUGg -3' miRNA: 3'- gGGGCUACCGgCaGUAUUGUCG--------UGAC- -5' |
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| 22414 | 5' | -53.8 | NC_005056.1 | + | 11004 | 0.67 | 0.576503 |
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Target: 5'- -gCCGGUGGCCGaccgCGUGACcGUGCa- -3' miRNA: 3'- ggGGCUACCGGCa---GUAUUGuCGUGac -5' |
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| 22414 | 5' | -53.8 | NC_005056.1 | + | 2989 | 0.68 | 0.531419 |
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Target: 5'- aCCCGc--GUCGUCA-GGCGGCGCUGc -3' miRNA: 3'- gGGGCuacCGGCAGUaUUGUCGUGAC- -5' |
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| 22414 | 5' | -53.8 | NC_005056.1 | + | 22379 | 0.69 | 0.487674 |
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Target: 5'- aCCCGuu-GCCGUCAgGGCAGCAa-- -3' miRNA: 3'- gGGGCuacCGGCAGUaUUGUCGUgac -5' |
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| 22414 | 5' | -53.8 | NC_005056.1 | + | 4020 | 0.69 | 0.466443 |
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Target: 5'- aCCgCCGGUGGCUuugCu--GCGGCGCUGc -3' miRNA: 3'- -GG-GGCUACCGGca-GuauUGUCGUGAC- -5' |
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| 22414 | 5' | -53.8 | NC_005056.1 | + | 11940 | 0.78 | 0.130398 |
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Target: 5'- aCCaCGAUGGCCGUCGggGAUGGCGgUGg -3' miRNA: 3'- gGG-GCUACCGGCAGUa-UUGUCGUgAC- -5' |
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| 22414 | 5' | -53.8 | NC_005056.1 | + | 19823 | 1.1 | 0.000681 |
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Target: 5'- gCCCCGAUGGCCGUCAUAACAGCACUGc -3' miRNA: 3'- -GGGGCUACCGGCAGUAUUGUCGUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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