Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22415 | 3' | -46.7 | NC_005056.1 | + | 17826 | 0.67 | 0.942105 |
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Target: 5'- uGGCCGGuaaGGUggUC-GGUUUUUugucGCCg -3' miRNA: 3'- -CUGGCC---CCGuuAGuCCAAAAAuu--UGG- -5' |
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| 22415 | 3' | -46.7 | NC_005056.1 | + | 19784 | 0.68 | 0.91691 |
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Target: 5'- cAUCGGGGCGAUUAgcuggccGGUUguu--GCCg -3' miRNA: 3'- cUGGCCCCGUUAGU-------CCAAaaauuUGG- -5' |
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| 22415 | 3' | -46.7 | NC_005056.1 | + | 21077 | 0.68 | 0.903317 |
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Target: 5'- aACCGGGGCuuUgAGGcu----GACCa -3' miRNA: 3'- cUGGCCCCGuuAgUCCaaaaauUUGG- -5' |
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| 22415 | 3' | -46.7 | NC_005056.1 | + | 28630 | 0.68 | 0.895682 |
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Target: 5'- --aCGGGGCGAa-AGGUUg--AAGCCc -3' miRNA: 3'- cugGCCCCGUUagUCCAAaaaUUUGG- -5' |
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| 22415 | 3' | -46.7 | NC_005056.1 | + | 10757 | 0.69 | 0.879444 |
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Target: 5'- uGCCGGGGUgauAAUCAGGcgugUGGugUg -3' miRNA: 3'- cUGGCCCCG---UUAGUCCaaaaAUUugG- -5' |
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| 22415 | 3' | -46.7 | NC_005056.1 | + | 20053 | 1.14 | 0.001844 |
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Target: 5'- uGACCGGGGCAAUCAGGUUUUUAAACCa -3' miRNA: 3'- -CUGGCCCCGUUAGUCCAAAAAUUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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