Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22416 | 5' | -45.2 | NC_005056.1 | + | 18764 | 0.67 | 0.978718 |
Target: 5'- uGCGGAGugcgGCCAuuucgGCGGAGUCuuuuGCCACg -3' miRNA: 3'- cCGUUUU----UGGU-----UGUCUUAGuc--UGGUG- -5' |
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22416 | 5' | -45.2 | NC_005056.1 | + | 2744 | 0.66 | 0.983716 |
Target: 5'- uGGCAcGggUCAACcGuuacAGUCAGACCGg -3' miRNA: 3'- -CCGUuUuuGGUUGuC----UUAGUCUGGUg -5' |
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22416 | 5' | -45.2 | NC_005056.1 | + | 23817 | 1.14 | 0.002893 |
Target: 5'- gGGCAAAAACCAACAGAAUCAGACCACu -3' miRNA: 3'- -CCGUUUUUGGUUGUCUUAGUCUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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