Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22417 | 3' | -52.6 | NC_005056.1 | + | 19014 | 0.66 | 0.693333 |
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Target: 5'- aGUGAUGCAcugacuuaucUCGGcCCGGuuGCGAagacgGCa -3' miRNA: 3'- gCACUACGU----------AGUCcGGCCuuUGCU-----CG- -5' |
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| 22417 | 3' | -52.6 | NC_005056.1 | + | 4975 | 0.67 | 0.65877 |
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Target: 5'- uCGUGAUgaGCAUCGcauccGCCGGGAaguACGGGa -3' miRNA: 3'- -GCACUA--CGUAGUc----CGGCCUU---UGCUCg -5' |
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| 22417 | 3' | -52.6 | NC_005056.1 | + | 21364 | 0.67 | 0.647177 |
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Target: 5'- gCGUGGUGuCAugccaggagccuUCAcGGCgGGAAuUGAGCg -3' miRNA: 3'- -GCACUAC-GU------------AGU-CCGgCCUUuGCUCG- -5' |
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| 22417 | 3' | -52.6 | NC_005056.1 | + | 8118 | 0.68 | 0.623957 |
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Target: 5'- gGUGGcacUGCGUCAGGaaCUGGAGaacgccGCGaAGCg -3' miRNA: 3'- gCACU---ACGUAGUCC--GGCCUU------UGC-UCG- -5' |
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| 22417 | 3' | -52.6 | NC_005056.1 | + | 20489 | 0.7 | 0.498994 |
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Target: 5'- cCGUcAUGCAUCAuGCUGGc-ACGGGCg -3' miRNA: 3'- -GCAcUACGUAGUcCGGCCuuUGCUCG- -5' |
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| 22417 | 3' | -52.6 | NC_005056.1 | + | 13533 | 0.7 | 0.477355 |
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Target: 5'- uCGUgGAUGUGUUAGaGCUGGAuAGCGAGa -3' miRNA: 3'- -GCA-CUACGUAGUC-CGGCCU-UUGCUCg -5' |
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| 22417 | 3' | -52.6 | NC_005056.1 | + | 10990 | 0.7 | 0.45619 |
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Target: 5'- gGUGAgGaCGUCAGGCCGGuggcCGAccGCg -3' miRNA: 3'- gCACUaC-GUAGUCCGGCCuuu-GCU--CG- -5' |
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| 22417 | 3' | -52.6 | NC_005056.1 | + | 6174 | 0.71 | 0.425421 |
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Target: 5'- gCGUGAgGCcaucaaGUCAGGCauggCGGAGACGAauGCg -3' miRNA: 3'- -GCACUaCG------UAGUCCG----GCCUUUGCU--CG- -5' |
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| 22417 | 3' | -52.6 | NC_005056.1 | + | 26142 | 1.12 | 0.000606 |
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Target: 5'- uCGUGAUGCAUCAGGCCGGAAACGAGCu -3' miRNA: 3'- -GCACUACGUAGUCCGGCCUUUGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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