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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22422 | 3' | -56.4 | NC_005056.1 | + | 10011 | 0.66 | 0.538034 |
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Target: 5'- aCCGcCG-GUAuGCGcGAUGUUGCcGCGCa -3' miRNA: 3'- aGGCcGCaCAU-CGC-CUAUAGCG-CGCG- -5' |
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| 22422 | 3' | -56.4 | NC_005056.1 | + | 2484 | 0.66 | 0.512057 |
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Target: 5'- aUCUGGCGggauuucaGGAUAUCGCGgaUGCg -3' miRNA: 3'- -AGGCCGCacaucg--CCUAUAGCGC--GCG- -5' |
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| 22422 | 3' | -56.4 | NC_005056.1 | + | 1588 | 0.67 | 0.474117 |
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Target: 5'- gUCGGCGUGUuccAGC-----UCGUGCGCu -3' miRNA: 3'- aGGCCGCACA---UCGccuauAGCGCGCG- -5' |
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| 22422 | 3' | -56.4 | NC_005056.1 | + | 18476 | 0.67 | 0.453656 |
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Target: 5'- aCCGGCagcGUGacgcgcUGGCGcGUGUCaGUGCGCa -3' miRNA: 3'- aGGCCG---CAC------AUCGCcUAUAG-CGCGCG- -5' |
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| 22422 | 3' | -56.4 | NC_005056.1 | + | 2127 | 0.68 | 0.414234 |
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Target: 5'- gCCaGCGUGgacggGGUGGAgaaauagGUCGaGCGCa -3' miRNA: 3'- aGGcCGCACa----UCGCCUa------UAGCgCGCG- -5' |
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| 22422 | 3' | -56.4 | NC_005056.1 | + | 10189 | 0.72 | 0.214385 |
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Target: 5'- aCCGGCGUGc-GCaGGAUAUCGC-CGa -3' miRNA: 3'- aGGCCGCACauCG-CCUAUAGCGcGCg -5' |
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| 22422 | 3' | -56.4 | NC_005056.1 | + | 11567 | 0.78 | 0.086608 |
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Target: 5'- aUCCGGCGUGUGGUGGAgc-CGCuCGg -3' miRNA: 3'- -AGGCCGCACAUCGCCUauaGCGcGCg -5' |
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| 22422 | 3' | -56.4 | NC_005056.1 | + | 1491 | 1.13 | 0.000231 |
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Target: 5'- uUCCGGCGUGUAGCGGAUAUCGCGCGCg -3' miRNA: 3'- -AGGCCGCACAUCGCCUAUAGCGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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