Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22422 | 5' | -54.5 | NC_005056.1 | + | 10134 | 0.66 | 0.571017 |
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Target: 5'- cGGCGAuauccuGCGCACGccggucGGCUcacGGGugAUGCg -3' miRNA: 3'- cUUGCU------CGCGUGC------UCGA---UCCugUGCG- -5' |
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| 22422 | 5' | -54.5 | NC_005056.1 | + | 11139 | 0.67 | 0.526163 |
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Target: 5'- -cACG-GCacaGCACcGGCUGGGGCGCGa -3' miRNA: 3'- cuUGCuCG---CGUGcUCGAUCCUGUGCg -5' |
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| 22422 | 5' | -54.5 | NC_005056.1 | + | 8045 | 0.67 | 0.515149 |
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Target: 5'- cAGCGAGgCGCGCaGGCUGGcGuuuuCAUGCu -3' miRNA: 3'- cUUGCUC-GCGUGcUCGAUC-Cu---GUGCG- -5' |
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| 22422 | 5' | -54.5 | NC_005056.1 | + | 6524 | 0.68 | 0.49341 |
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Target: 5'- -cACGAuGCaGCGCG-GCUuguccuGGAUACGCg -3' miRNA: 3'- cuUGCU-CG-CGUGCuCGAu-----CCUGUGCG- -5' |
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| 22422 | 5' | -54.5 | NC_005056.1 | + | 2891 | 0.68 | 0.461624 |
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Target: 5'- aAAC-AGCGCgACG-GCUGGGACAgCGUu -3' miRNA: 3'- cUUGcUCGCG-UGCuCGAUCCUGU-GCG- -5' |
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| 22422 | 5' | -54.5 | NC_005056.1 | + | 10623 | 0.69 | 0.441043 |
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Target: 5'- uGAAUGAGgcUGCACGGGC--GGugAUGCu -3' miRNA: 3'- -CUUGCUC--GCGUGCUCGauCCugUGCG- -5' |
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| 22422 | 5' | -54.5 | NC_005056.1 | + | 8776 | 0.69 | 0.43095 |
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Target: 5'- uGAcCGAGCGCACGcuggucAGUgagguGGACgGCGCa -3' miRNA: 3'- -CUuGCUCGCGUGC------UCGau---CCUG-UGCG- -5' |
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| 22422 | 5' | -54.5 | NC_005056.1 | + | 16500 | 0.72 | 0.267546 |
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Target: 5'- uAGCGcgcagacccAGCGCACGGGCgguGGcAUACGCg -3' miRNA: 3'- cUUGC---------UCGCGUGCUCGau-CC-UGUGCG- -5' |
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| 22422 | 5' | -54.5 | NC_005056.1 | + | 5358 | 0.73 | 0.246851 |
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Target: 5'- uGAACGGcGUGCugGAGC-AGGGCAaaggUGCa -3' miRNA: 3'- -CUUGCU-CGCGugCUCGaUCCUGU----GCG- -5' |
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| 22422 | 5' | -54.5 | NC_005056.1 | + | 1526 | 0.98 | 0.004036 |
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Target: 5'- aGAACGAGCGCACGAGCUGGaACACGCc -3' miRNA: 3'- -CUUGCUCGCGUGCUCGAUCcUGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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