Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22424 | 3' | -55.9 | NC_005056.1 | + | 10761 | 0.66 | 0.454307 |
Target: 5'- cAUCuGCCGGGguGauaaucAGGCGuGUGGUGUg -3' miRNA: 3'- uUGGuCGGCCCguU------UCCGC-UACUACA- -5' |
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22424 | 3' | -55.9 | NC_005056.1 | + | 8963 | 0.66 | 0.486192 |
Target: 5'- cACC-GUCaGGGCGucGGCGAUGA-GUg -3' miRNA: 3'- uUGGuCGG-CCCGUuuCCGCUACUaCA- -5' |
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22424 | 3' | -55.9 | NC_005056.1 | + | 7746 | 0.66 | 0.453263 |
Target: 5'- cAGCCGGUgccguggcgcagaCGGGCGGcggcucuGGUGGUGAUGg -3' miRNA: 3'- -UUGGUCG-------------GCCCGUUu------CCGCUACUACa -5' |
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22424 | 3' | -55.9 | NC_005056.1 | + | 6210 | 0.66 | 0.486192 |
Target: 5'- cACgCAGaCGGGCGGGGGCGAcGGUc- -3' miRNA: 3'- uUG-GUCgGCCCGUUUCCGCUaCUAca -5' |
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22424 | 3' | -55.9 | NC_005056.1 | + | 2347 | 1.05 | 0.000599 |
Target: 5'- cAACCAGCCGGGCAAAGGCGAUGAUGUa -3' miRNA: 3'- -UUGGUCGGCCCGUUUCCGCUACUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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