Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22425 | 5' | -57.1 | NC_005056.1 | + | 20417 | 0.66 | 0.435395 |
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Target: 5'- uGGcGgUCAAGCuguguccCCGGCGCaGUACCAc -3' miRNA: 3'- -CCaCgAGUUCG-------GGCCGCGcUAUGGUc -5' |
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| 22425 | 5' | -57.1 | NC_005056.1 | + | 1657 | 0.7 | 0.249113 |
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Target: 5'- uGGUgacGCUCAAGUauucugaauuugccaCCGGCcaccaccgGCGGUGCCAGu -3' miRNA: 3'- -CCA---CGAGUUCG---------------GGCCG--------CGCUAUGGUC- -5' |
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| 22425 | 5' | -57.1 | NC_005056.1 | + | 23789 | 0.72 | 0.187948 |
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Target: 5'- uGUGUgCGAGagCCGGCGCGAUcugACCAGc -3' miRNA: 3'- cCACGaGUUCg-GGCCGCGCUA---UGGUC- -5' |
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| 22425 | 5' | -57.1 | NC_005056.1 | + | 9628 | 0.77 | 0.090511 |
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Target: 5'- uGUGCgCGAacGCCCGGCGCGAUgggucagccacugaaGCCAGu -3' miRNA: 3'- cCACGaGUU--CGGGCCGCGCUA---------------UGGUC- -5' |
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| 22425 | 5' | -57.1 | NC_005056.1 | + | 2514 | 1.1 | 0.000258 |
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Target: 5'- cGGUGCUCAAGCCCGGCGCGAUACCAGu -3' miRNA: 3'- -CCACGAGUUCGGGCCGCGCUAUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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