Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22427 | 3' | -53.1 | NC_005056.1 | + | 8901 | 0.66 | 0.677994 |
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Target: 5'- -cACcGGGCGGGUGAccggcuCCGGcggCGGGg -3' miRNA: 3'- acUGuCCCGCCUACUuu----GGUCa--GUCC- -5' |
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| 22427 | 3' | -53.1 | NC_005056.1 | + | 11917 | 0.67 | 0.654944 |
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Target: 5'- cGGgAGGGCGGAaGGucaacauuACCAcgauggccGUCGGGg -3' miRNA: 3'- aCUgUCCCGCCUaCUu-------UGGU--------CAGUCC- -5' |
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| 22427 | 3' | -53.1 | NC_005056.1 | + | 13778 | 0.69 | 0.528888 |
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Target: 5'- uUGAU--GGCGauGAUGAAGCCAucGUCAGGc -3' miRNA: 3'- -ACUGucCCGC--CUACUUUGGU--CAGUCC- -5' |
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| 22427 | 3' | -53.1 | NC_005056.1 | + | 2135 | 0.7 | 0.474319 |
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Target: 5'- gGACGGGGUGGA-GAAAUaGGUCGa- -3' miRNA: 3'- aCUGUCCCGCCUaCUUUGgUCAGUcc -5' |
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| 22427 | 3' | -53.1 | NC_005056.1 | + | 5914 | 0.7 | 0.46374 |
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Target: 5'- cGcCAGGGCGGcac--GCCGGUCAGu -3' miRNA: 3'- aCuGUCCCGCCuacuuUGGUCAGUCc -5' |
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| 22427 | 3' | -53.1 | NC_005056.1 | + | 9739 | 0.7 | 0.44296 |
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Target: 5'- cUGACGGGGCGGugauUGAguaugaacccgaAACCAGUgCAc- -3' miRNA: 3'- -ACUGUCCCGCCu---ACU------------UUGGUCA-GUcc -5' |
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| 22427 | 3' | -53.1 | NC_005056.1 | + | 3886 | 1.1 | 0.00074 |
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Target: 5'- cUGACAGGGCGGAUGAAACCAGUCAGGc -3' miRNA: 3'- -ACUGUCCCGCCUACUUUGGUCAGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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