Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22427 | 5' | -49.6 | NC_005056.1 | + | 16887 | 0.66 | 0.880237 |
Target: 5'- gGCC-GGAAAACuGUauaUCGACUACGa -3' miRNA: 3'- gCGGaCUUUUUGuCAg--AGCUGGUGUg -5' |
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22427 | 5' | -49.6 | NC_005056.1 | + | 6628 | 0.67 | 0.835485 |
Target: 5'- gGCgaGc---ACGGcCUCGACCACGCc -3' miRNA: 3'- gCGgaCuuuuUGUCaGAGCUGGUGUG- -5' |
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22427 | 5' | -49.6 | NC_005056.1 | + | 27547 | 0.67 | 0.825739 |
Target: 5'- uGCCUG-AAAACGGUUUCGcGCgGCu- -3' miRNA: 3'- gCGGACuUUUUGUCAGAGC-UGgUGug -5' |
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22427 | 5' | -49.6 | NC_005056.1 | + | 5788 | 0.67 | 0.825739 |
Target: 5'- aCGCCUGccuGACAGUCgagcugUG-UCGCACg -3' miRNA: 3'- -GCGGACuuuUUGUCAGa-----GCuGGUGUG- -5' |
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22427 | 5' | -49.6 | NC_005056.1 | + | 25396 | 0.67 | 0.815754 |
Target: 5'- -uCCUGAcuGAGCAG-CUCGAcuaucuCCACGCg -3' miRNA: 3'- gcGGACUu-UUUGUCaGAGCU------GGUGUG- -5' |
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22427 | 5' | -49.6 | NC_005056.1 | + | 12425 | 0.69 | 0.728872 |
Target: 5'- aCGCC--GAAAGCGGUUaaGGCCGCAUa -3' miRNA: 3'- -GCGGacUUUUUGUCAGagCUGGUGUG- -5' |
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22427 | 5' | -49.6 | NC_005056.1 | + | 20360 | 0.7 | 0.670412 |
Target: 5'- gCGCCgGGGAcACAG-CUUGACCGC-Ca -3' miRNA: 3'- -GCGGaCUUUuUGUCaGAGCUGGUGuG- -5' |
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22427 | 5' | -49.6 | NC_005056.1 | + | 5470 | 0.72 | 0.51727 |
Target: 5'- uGcCCUGAAGuACGGUCU-GACCAUGCc -3' miRNA: 3'- gC-GGACUUUuUGUCAGAgCUGGUGUG- -5' |
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22427 | 5' | -49.6 | NC_005056.1 | + | 703 | 0.73 | 0.483573 |
Target: 5'- uGCCUGGcuGAAugAG--UCGGCCACGCu -3' miRNA: 3'- gCGGACU--UUUugUCagAGCUGGUGUG- -5' |
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22427 | 5' | -49.6 | NC_005056.1 | + | 3920 | 1.1 | 0.001698 |
Target: 5'- cCGCCUGAAAAACAGUCUCGACCACACc -3' miRNA: 3'- -GCGGACUUUUUGUCAGAGCUGGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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