Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22428 | 3' | -58.1 | NC_005056.1 | + | 19879 | 0.66 | 0.394026 |
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Target: 5'- uAAGCaaCGCCCCG--GCGACAaugGCGGCc -3' miRNA: 3'- cUUCG--GCGGGGCgaCGCUGUa--CGUUG- -5' |
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| 22428 | 3' | -58.1 | NC_005056.1 | + | 25251 | 0.66 | 0.366475 |
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Target: 5'- cGAAGCacagGCCgaCGCUGCGACGcGCu-- -3' miRNA: 3'- -CUUCGg---CGGg-GCGACGCUGUaCGuug -5' |
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| 22428 | 3' | -58.1 | NC_005056.1 | + | 164 | 0.66 | 0.348859 |
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Target: 5'- aAAGCCGCCCgcagGCgGCGAUGUuCAGCc -3' miRNA: 3'- cUUCGGCGGGg---CGaCGCUGUAcGUUG- -5' |
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| 22428 | 3' | -58.1 | NC_005056.1 | + | 7811 | 0.67 | 0.34028 |
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Target: 5'- aGAGCCGCcgCCCGuCUGCGcCAcgGCAc- -3' miRNA: 3'- cUUCGGCG--GGGC-GACGCuGUa-CGUug -5' |
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| 22428 | 3' | -58.1 | NC_005056.1 | + | 1352 | 0.67 | 0.307507 |
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Target: 5'- --cGCCGaCCUCGCcuggGCGAcCAUGCAc- -3' miRNA: 3'- cuuCGGC-GGGGCGa---CGCU-GUACGUug -5' |
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| 22428 | 3' | -58.1 | NC_005056.1 | + | 11187 | 0.7 | 0.189722 |
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Target: 5'- --uGCCGCUuuGCaUGUggaaGGCGUGCAGCg -3' miRNA: 3'- cuuCGGCGGggCG-ACG----CUGUACGUUG- -5' |
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| 22428 | 3' | -58.1 | NC_005056.1 | + | 24696 | 0.72 | 0.14716 |
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Target: 5'- -uGGCCGuCCCuCGCUGgcaggaGACAUGCGAUu -3' miRNA: 3'- cuUCGGC-GGG-GCGACg-----CUGUACGUUG- -5' |
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| 22428 | 3' | -58.1 | NC_005056.1 | + | 22371 | 0.73 | 0.120275 |
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Target: 5'- aAGGCCGCaCCCGUUGCcGuCAggGCAGCa -3' miRNA: 3'- cUUCGGCG-GGGCGACG-CuGUa-CGUUG- -5' |
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| 22428 | 3' | -58.1 | NC_005056.1 | + | 5212 | 1.08 | 0.000223 |
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Target: 5'- gGAAGCCGCCCCGCUGCGACAUGCAACu -3' miRNA: 3'- -CUUCGGCGGGGCGACGCUGUACGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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