Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22428 | 5' | -55.8 | NC_005056.1 | + | 18153 | 0.66 | 0.500233 |
Target: 5'- cGGUcAGGCaUCccguauugagggauuCCGCaAGACCAGUGCa -3' miRNA: 3'- aCCAaUUCG-AGc--------------GGCG-UCUGGUCGCG- -5' |
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22428 | 5' | -55.8 | NC_005056.1 | + | 16511 | 0.66 | 0.488407 |
Target: 5'- cUGGUcGAuuUUaGCgCGCAGACCcAGCGCa -3' miRNA: 3'- -ACCAaUUcgAG-CG-GCGUCUGG-UCGCG- -5' |
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22428 | 5' | -55.8 | NC_005056.1 | + | 6583 | 0.66 | 0.487338 |
Target: 5'- aGGccguGCUCGCCGCaaauccgGGACUGGCu- -3' miRNA: 3'- aCCaauuCGAGCGGCG-------UCUGGUCGcg -5' |
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22428 | 5' | -55.8 | NC_005056.1 | + | 4579 | 0.67 | 0.460995 |
Target: 5'- cGGUc-GGCUgGCUGCAGaaauaccgcaaugaaGCCccGGCGCg -3' miRNA: 3'- aCCAauUCGAgCGGCGUC---------------UGG--UCGCG- -5' |
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22428 | 5' | -55.8 | NC_005056.1 | + | 9515 | 0.67 | 0.446576 |
Target: 5'- cGGUc--GCU-GCCguguGCAGACCGGCGg -3' miRNA: 3'- aCCAauuCGAgCGG----CGUCUGGUCGCg -5' |
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22428 | 5' | -55.8 | NC_005056.1 | + | 5752 | 0.67 | 0.426422 |
Target: 5'- aGG-UGAGCcagCGCCcgGCGGcaucACCAGCGUc -3' miRNA: 3'- aCCaAUUCGa--GCGG--CGUC----UGGUCGCG- -5' |
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22428 | 5' | -55.8 | NC_005056.1 | + | 10078 | 0.68 | 0.39723 |
Target: 5'- cGGUUGuGC-CGCCGCuGucGCCGGgGUg -3' miRNA: 3'- aCCAAUuCGaGCGGCGuC--UGGUCgCG- -5' |
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22428 | 5' | -55.8 | NC_005056.1 | + | 17237 | 0.69 | 0.360365 |
Target: 5'- cUGG--GAGCUuuaCGCCGCA-ACCGGUGUg -3' miRNA: 3'- -ACCaaUUCGA---GCGGCGUcUGGUCGCG- -5' |
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22428 | 5' | -55.8 | NC_005056.1 | + | 10185 | 0.7 | 0.28421 |
Target: 5'- cGGUUcguugcuGGCgucaaugauugaccaGCCGCAGACCccGGCGCu -3' miRNA: 3'- aCCAAu------UCGag-------------CGGCGUCUGG--UCGCG- -5' |
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22428 | 5' | -55.8 | NC_005056.1 | + | 5255 | 1.12 | 0.000229 |
Target: 5'- cUGGUUAAGCUCGCCGCAGACCAGCGCa -3' miRNA: 3'- -ACCAAUUCGAGCGGCGUCUGGUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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