miRNA: 3'- -GCGACG------GCCACUCAGACaCCUAU---GGG- -5'
miRNA: 3'- -GCGACGGCCACuCagacaCCUAugGg -5'
miRNA: 3'- gCGACGGCCACU-CAGa--CACcUAUGGg -5'
miRNA: 3'- -GCGACGGCCAc--UCAG-ACaCCUAUgGG- -5'
miRNA: 3'- -GCGACGGCCACUCAGACACCUAUGGG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22429 | 3' | -55.8 | NC_005056.1 | + | 1591 | 0.67 | 0.486808 |
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Target: 5'- aCGUUGUauuuuuCGGUGAGUUUGcGGAUGgauUCCg -3' miRNA: 3'- -GCGACG------GCCACUCAGACaCCUAU---GGG- -5' |
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| 22429 | 3' | -55.8 | NC_005056.1 | + | 18587 | 0.69 | 0.387445 |
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Target: 5'- gCGCUGCCGGUGuGg----GGAUugCg -3' miRNA: 3'- -GCGACGGCCACuCagacaCCUAugGg -5' |
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| 22429 | 3' | -55.8 | NC_005056.1 | + | 7637 | 0.7 | 0.302383 |
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Target: 5'- gGUUGCCGGUGGuGUCggcGUGuAUGCCa -3' miRNA: 3'- gCGACGGCCACU-CAGa--CACcUAUGGg -5' |
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| 22429 | 3' | -55.8 | NC_005056.1 | + | 1366 | 0.71 | 0.287199 |
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Target: 5'- cCGCUGCUGGUcauGGUCuUGcGGAUAgCCa -3' miRNA: 3'- -GCGACGGCCAc--UCAG-ACaCCUAUgGG- -5' |
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| 22429 | 3' | -55.8 | NC_005056.1 | + | 5565 | 1.13 | 0.000234 |
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Target: 5'- aCGCUGCCGGUGAGUCUGUGGAUACCCg -3' miRNA: 3'- -GCGACGGCCACUCAGACACCUAUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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