Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22430 | 5' | -53.3 | NC_005056.1 | + | 3977 | 0.7 | 0.462444 |
Target: 5'- --aGcCACCGGCGGuGGCGUG-ACGCCc -3' miRNA: 3'- gcaCuGUGGCCGUU-UUGCACcUGCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 4366 | 0.7 | 0.451099 |
Target: 5'- gGUGcCACCGGCGGugucgguggugcuGGCGaUGGA-GCCg -3' miRNA: 3'- gCACuGUGGCCGUU-------------UUGC-ACCUgCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 7554 | 0.7 | 0.438898 |
Target: 5'- -cUGGCACUGucccugauuuacccGCAGAGCGUGGcCGUCa -3' miRNA: 3'- gcACUGUGGC--------------CGUUUUGCACCuGCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 7460 | 0.71 | 0.421942 |
Target: 5'- aCGcUGACcguaACCGGCAcc-CGgcagGGACGCCc -3' miRNA: 3'- -GC-ACUG----UGGCCGUuuuGCa---CCUGCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 5613 | 0.78 | 0.163724 |
Target: 5'- --aGACucACCGGCA--GCGUGGGCGCg -3' miRNA: 3'- gcaCUG--UGGCCGUuuUGCACCUGCGg -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 17300 | 0.82 | 0.083697 |
Target: 5'- gCGUGACgauACCGGCGAAACGgUGGcCGUCg -3' miRNA: 3'- -GCACUG---UGGCCGUUUUGC-ACCuGCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 5850 | 1.12 | 0.00064 |
Target: 5'- gCGUGACACCGGCAAAACGUGGACGCCc -3' miRNA: 3'- -GCACUGUGGCCGUUUUGCACCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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