miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22430 5' -53.3 NC_005056.1 + 3977 0.7 0.462444
Target:  5'- --aGcCACCGGCGGuGGCGUG-ACGCCc -3'
miRNA:   3'- gcaCuGUGGCCGUU-UUGCACcUGCGG- -5'
22430 5' -53.3 NC_005056.1 + 4366 0.7 0.451099
Target:  5'- gGUGcCACCGGCGGugucgguggugcuGGCGaUGGA-GCCg -3'
miRNA:   3'- gCACuGUGGCCGUU-------------UUGC-ACCUgCGG- -5'
22430 5' -53.3 NC_005056.1 + 7554 0.7 0.438898
Target:  5'- -cUGGCACUGucccugauuuacccGCAGAGCGUGGcCGUCa -3'
miRNA:   3'- gcACUGUGGC--------------CGUUUUGCACCuGCGG- -5'
22430 5' -53.3 NC_005056.1 + 7460 0.71 0.421942
Target:  5'- aCGcUGACcguaACCGGCAcc-CGgcagGGACGCCc -3'
miRNA:   3'- -GC-ACUG----UGGCCGUuuuGCa---CCUGCGG- -5'
22430 5' -53.3 NC_005056.1 + 5613 0.78 0.163724
Target:  5'- --aGACucACCGGCA--GCGUGGGCGCg -3'
miRNA:   3'- gcaCUG--UGGCCGUuuUGCACCUGCGg -5'
22430 5' -53.3 NC_005056.1 + 17300 0.82 0.083697
Target:  5'- gCGUGACgauACCGGCGAAACGgUGGcCGUCg -3'
miRNA:   3'- -GCACUG---UGGCCGUUUUGC-ACCuGCGG- -5'
22430 5' -53.3 NC_005056.1 + 5850 1.12 0.00064
Target:  5'- gCGUGACACCGGCAAAACGUGGACGCCc -3'
miRNA:   3'- -GCACUGUGGCCGUUUUGCACCUGCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.