miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22431 3' -65.5 NC_005056.1 + 10021 0.66 0.170308
Target:  5'- cCCCgGCGaCAGcGGCGGCACaaCCGGg-- -3'
miRNA:   3'- -GGG-CGCgGUC-CCGCCGUGc-GGCCagu -5'
22431 3' -65.5 NC_005056.1 + 6020 0.66 0.18907
Target:  5'- cUCUGaaaaGUC-GGGCGGUggACGCCGGUaCAg -3'
miRNA:   3'- -GGGCg---CGGuCCCGCCG--UGCGGCCA-GU- -5'
22431 3' -65.5 NC_005056.1 + 10407 0.66 0.18907
Target:  5'- gCUG-GCCGGuaucGGUGuGCugGCCGGUUAa -3'
miRNA:   3'- gGGCgCGGUC----CCGC-CGugCGGCCAGU- -5'
22431 3' -65.5 NC_005056.1 + 20265 0.66 0.179472
Target:  5'- aCCgGUGCCAuacGuGGCGGCauuaguccuuGCGgCGGUCu -3'
miRNA:   3'- -GGgCGCGGU---C-CCGCCG----------UGCgGCCAGu -5'
22431 3' -65.5 NC_005056.1 + 2989 0.66 0.165885
Target:  5'- aCCCGCGUCGucaGGCGGCGCuGCUGu--- -3'
miRNA:   3'- -GGGCGCGGUc--CCGCCGUG-CGGCcagu -5'
22431 3' -65.5 NC_005056.1 + 10590 0.67 0.149208
Target:  5'- uUCCaGCGUCAGGGUacgGGCGaccgccuccUGCUGGUCu -3'
miRNA:   3'- -GGG-CGCGGUCCCG---CCGU---------GCGGCCAGu -5'
22431 3' -65.5 NC_005056.1 + 11910 0.68 0.12366
Target:  5'- aCCGCGCCGGGaG-GGCG-GaaGGUCAa -3'
miRNA:   3'- gGGCGCGGUCC-CgCCGUgCggCCAGU- -5'
22431 3' -65.5 NC_005056.1 + 18533 0.68 0.134071
Target:  5'- -aCGCGCCAGcGCGuCACgcuGCCGGUUAa -3'
miRNA:   3'- ggGCGCGGUCcCGCcGUG---CGGCCAGU- -5'
22431 3' -65.5 NC_005056.1 + 10264 0.68 0.117141
Target:  5'- --aGCGCCGGGGUcuGCG-GCUGGUCAa -3'
miRNA:   3'- gggCGCGGUCCCGc-CGUgCGGCCAGU- -5'
22431 3' -65.5 NC_005056.1 + 18823 0.73 0.046884
Target:  5'- gCUGCgGCUAuucaGGCGGCaACGCCGGUCAc -3'
miRNA:   3'- gGGCG-CGGUc---CCGCCG-UGCGGCCAGU- -5'
22431 3' -65.5 NC_005056.1 + 5909 1.09 5.7e-05
Target:  5'- cCCCGCGCCAGGGCGGCACGCCGGUCAg -3'
miRNA:   3'- -GGGCGCGGUCCCGCCGUGCGGCCAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.