Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22436 | 3' | -52.3 | NC_005056.1 | + | 18582 | 0.66 | 0.69967 |
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Target: 5'- ---aGUgGgCGCUGCCGGUGUGGg-- -3' miRNA: 3'- gcaaUAgUgGUGACGGCCACGUCauu -5' |
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| 22436 | 3' | -52.3 | NC_005056.1 | + | 6126 | 0.66 | 0.69967 |
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Target: 5'- uGUUuuuaAUCACCGgcucgucgcCUGCCGGUuGCGGg-- -3' miRNA: 3'- gCAA----UAGUGGU---------GACGGCCA-CGUCauu -5' |
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| 22436 | 3' | -52.3 | NC_005056.1 | + | 6764 | 0.66 | 0.688003 |
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Target: 5'- uGUUGUCGgCAaggugcUUGCCGGUGguGa-- -3' miRNA: 3'- gCAAUAGUgGU------GACGGCCACguCauu -5' |
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| 22436 | 3' | -52.3 | NC_005056.1 | + | 9827 | 0.67 | 0.617171 |
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Target: 5'- uGUUAcUGCCACggUGCCGGUGgucaCGGUGAa -3' miRNA: 3'- gCAAUaGUGGUG--ACGGCCAC----GUCAUU- -5' |
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| 22436 | 3' | -52.3 | NC_005056.1 | + | 10077 | 0.67 | 0.613621 |
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Target: 5'- gGUUGUgcCGCCGCUgucGCCGGgguguuuauggguaUGCAGUAc -3' miRNA: 3'- gCAAUA--GUGGUGA---CGGCC--------------ACGUCAUu -5' |
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| 22436 | 3' | -52.3 | NC_005056.1 | + | 7625 | 0.68 | 0.593548 |
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Target: 5'- gGUUAUCugUGuggUUGCCGGUGguGUc- -3' miRNA: 3'- gCAAUAGugGU---GACGGCCACguCAuu -5' |
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| 22436 | 3' | -52.3 | NC_005056.1 | + | 22272 | 0.68 | 0.546839 |
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Target: 5'- gCGUUAUCAUCAgUGCCaGaacgugaauUGCAGUGc -3' miRNA: 3'- -GCAAUAGUGGUgACGGcC---------ACGUCAUu -5' |
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| 22436 | 3' | -52.3 | NC_005056.1 | + | 17848 | 0.7 | 0.457619 |
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Target: 5'- -uUUGUCGCCGaacUCGGUGCAGUGAc -3' miRNA: 3'- gcAAUAGUGGUgacGGCCACGUCAUU- -5' |
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| 22436 | 3' | -52.3 | NC_005056.1 | + | 7944 | 0.7 | 0.426193 |
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Target: 5'- uGUUGUUAUCGCUGgCGGUGgCGGg-- -3' miRNA: 3'- gCAAUAGUGGUGACgGCCAC-GUCauu -5' |
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| 22436 | 3' | -52.3 | NC_005056.1 | + | 11869 | 1.07 | 0.001185 |
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Target: 5'- cCGUUAUCACCACUGCCGGUGCAGUAAa -3' miRNA: 3'- -GCAAUAGUGGUGACGGCCACGUCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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