miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22439 3' -62.5 NC_005056.1 + 19646 0.66 0.232249
Target:  5'- cGCACUgGCGCUgacugGCGUcaucGGUGCcauUGGCCu -3'
miRNA:   3'- -UGUGGgCGUGG-----CGCGu---CCACG---ACCGG- -5'
22439 3' -62.5 NC_005056.1 + 1715 0.66 0.238358
Target:  5'- cGCAUCCgGCGCUGuCGCcacGGUGUgacgGGUCg -3'
miRNA:   3'- -UGUGGG-CGUGGC-GCGu--CCACGa---CCGG- -5'
22439 3' -62.5 NC_005056.1 + 8906 0.66 0.2446
Target:  5'- -aGCUC-CACCGgGCGGGUGaCcGGCUc -3'
miRNA:   3'- ugUGGGcGUGGCgCGUCCAC-GaCCGG- -5'
22439 3' -62.5 NC_005056.1 + 9880 0.66 0.238358
Target:  5'- cCACCgGCACCGUGgCAguaacagaaccGGaagcugucacGCUGGCCg -3'
miRNA:   3'- uGUGGgCGUGGCGC-GU-----------CCa---------CGACCGG- -5'
22439 3' -62.5 NC_005056.1 + 3510 0.67 0.209122
Target:  5'- aACACCgGCAaauguuauCUG-GUGGG-GCUGGCCg -3'
miRNA:   3'- -UGUGGgCGU--------GGCgCGUCCaCGACCGG- -5'
22439 3' -62.5 NC_005056.1 + 15 0.68 0.168795
Target:  5'- aGCACUgCGCGCUgGCGguGGUGCUGa-- -3'
miRNA:   3'- -UGUGG-GCGUGG-CGCguCCACGACcgg -5'
22439 3' -62.5 NC_005056.1 + 8455 0.68 0.183032
Target:  5'- -aACCCGacaGCCuGCGCAGGgcGCUGacugauGCCg -3'
miRNA:   3'- ugUGGGCg--UGG-CGCGUCCa-CGAC------CGG- -5'
22439 3' -62.5 NC_005056.1 + 8631 0.71 0.108461
Target:  5'- -gACCUGCucAUUGUGCcGGUGCUGGCg -3'
miRNA:   3'- ugUGGGCG--UGGCGCGuCCACGACCGg -5'
22439 3' -62.5 NC_005056.1 + 1744 0.71 0.108461
Target:  5'- gUACUgGCACCGC-C-GGUGgUGGCCg -3'
miRNA:   3'- uGUGGgCGUGGCGcGuCCACgACCGG- -5'
22439 3' -62.5 NC_005056.1 + 16740 0.71 0.096893
Target:  5'- aGCACCUGCGCgGUGCGGGUGUa---- -3'
miRNA:   3'- -UGUGGGCGUGgCGCGUCCACGaccgg -5'
22439 3' -62.5 NC_005056.1 + 4362 0.74 0.059581
Target:  5'- cCACCgGCGgUGuCGguGGUGCUGGCg -3'
miRNA:   3'- uGUGGgCGUgGC-GCguCCACGACCGg -5'
22439 3' -62.5 NC_005056.1 + 16687 1.11 6.7e-05
Target:  5'- uACACCCGCACCGCGCAGGUGCUGGCCg -3'
miRNA:   3'- -UGUGGGCGUGGCGCGUCCACGACCGG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.