Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2244 | 3' | -57.3 | NC_001405.1 | + | 9837 | 0.66 | 0.516626 |
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Target: 5'- aUGUCCUUGGGUccGGccUGCUgaaugcgcagGCGGUCg -3' miRNA: 3'- -ACAGGAGCUCG--CCaaACGG----------CGCCAGg -5' |
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| 2244 | 3' | -57.3 | NC_001405.1 | + | 16314 | 0.68 | 0.366416 |
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Target: 5'- gGUCCaggcgaCGAGCGGccgccgcagcaGCCGCGG-CCa -3' miRNA: 3'- aCAGGa-----GCUCGCCaaa--------CGGCGCCaGG- -5' |
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| 2244 | 3' | -57.3 | NC_001405.1 | + | 15171 | 0.69 | 0.352614 |
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Target: 5'- aUGUCCUCcacCGGcugcaUUGCCGCGGcugCCg -3' miRNA: 3'- -ACAGGAGcucGCCa----AACGGCGCCa--GG- -5' |
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| 2244 | 3' | -57.3 | NC_001405.1 | + | 8515 | 0.75 | 0.144059 |
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Target: 5'- gGUaCCgcgcggCGGGCGGUggGCCGCGGgggugUCCu -3' miRNA: 3'- aCA-GGa-----GCUCGCCAaaCGGCGCC-----AGG- -5' |
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| 2244 | 3' | -57.3 | NC_001405.1 | + | 17249 | 0.76 | 0.105549 |
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Target: 5'- uUG-CCUCG-GCGGUggcagaUGCCGCGGUgCa -3' miRNA: 3'- -ACaGGAGCuCGCCAa-----ACGGCGCCAgG- -5' |
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| 2244 | 3' | -57.3 | NC_001405.1 | + | 5768 | 0.96 | 0.004002 |
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Target: 5'- cUGUCCUCGAGCGGUguuCCGCGGUCCu -3' miRNA: 3'- -ACAGGAGCUCGCCAaacGGCGCCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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