miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22441 3' -56.2 NC_005056.1 + 25909 0.66 0.482012
Target:  5'- -cGCGUG-GCUGAcggauaagcugcacgACGGgcagCAGGUAUUg -3'
miRNA:   3'- gaCGCGCuCGACU---------------UGCCa---GUCCGUAG- -5'
22441 3' -56.2 NC_005056.1 + 12558 0.67 0.45067
Target:  5'- -cGCGCuacuGAGCUGGacaaggccuuuucgcGCGGUgGuGGCGUCc -3'
miRNA:   3'- gaCGCG----CUCGACU---------------UGCCAgU-CCGUAG- -5'
22441 3' -56.2 NC_005056.1 + 17070 0.67 0.446576
Target:  5'- uUGCGCG-GCauUGccauCGGUCAGGC-UCc -3'
miRNA:   3'- gACGCGCuCG--ACuu--GCCAGUCCGuAG- -5'
22441 3' -56.2 NC_005056.1 + 16570 0.67 0.436432
Target:  5'- -gGCacCGAuGCUGAccugcuuaACGaGUCAGGCGUCa -3'
miRNA:   3'- gaCGc-GCU-CGACU--------UGC-CAGUCCGUAG- -5'
22441 3' -56.2 NC_005056.1 + 16364 0.7 0.294043
Target:  5'- -aGCGUGAGCUGA-UGGUCAucuGGCc-- -3'
miRNA:   3'- gaCGCGCUCGACUuGCCAGU---CCGuag -5'
22441 3' -56.2 NC_005056.1 + 6708 0.71 0.237632
Target:  5'- -gGCGCGGuCUGAAC-GUCGGGCAguUCg -3'
miRNA:   3'- gaCGCGCUcGACUUGcCAGUCCGU--AG- -5'
22441 3' -56.2 NC_005056.1 + 18138 1.09 0.000354
Target:  5'- aCUGCGCGAGCUGAACGGUCAGGCAUCc -3'
miRNA:   3'- -GACGCGCUCGACUUGCCAGUCCGUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.