Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22443 | 3' | -53.6 | NC_005056.1 | + | 23195 | 0.66 | 0.623382 |
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Target: 5'- --uGGcCUCCGCC--AGUGGCgGCAg -3' miRNA: 3'- uuuUCuGAGGCGGcuUUACCGgCGUg -5' |
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| 22443 | 3' | -53.6 | NC_005056.1 | + | 1619 | 0.66 | 0.611627 |
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Target: 5'- --uGGAUUCCGCCuGAGUGGCaaaGUcCa -3' miRNA: 3'- uuuUCUGAGGCGGcUUUACCGg--CGuG- -5' |
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| 22443 | 3' | -53.6 | NC_005056.1 | + | 13220 | 0.66 | 0.611627 |
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Target: 5'- cAAAAGACcugaugaCGCUGAAAUGGgaUGCACu -3' miRNA: 3'- -UUUUCUGag-----GCGGCUUUACCg-GCGUG- -5' |
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| 22443 | 3' | -53.6 | NC_005056.1 | + | 9453 | 0.66 | 0.588187 |
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Target: 5'- gGAAGGAUgCCGCCGGGucauaccGGCgGCAa -3' miRNA: 3'- -UUUUCUGaGGCGGCUUua-----CCGgCGUg -5' |
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| 22443 | 3' | -53.6 | NC_005056.1 | + | 22352 | 0.66 | 0.576525 |
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Target: 5'- cGGGAGAaguccaCGCCGuaa-GGCCGCACc -3' miRNA: 3'- -UUUUCUgag---GCGGCuuuaCCGGCGUG- -5' |
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| 22443 | 3' | -53.6 | NC_005056.1 | + | 11201 | 0.7 | 0.364436 |
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Target: 5'- uGGAAGGCgugcagCgUGUCGAGcUGGCCGCACc -3' miRNA: 3'- -UUUUCUGa-----G-GCGGCUUuACCGGCGUG- -5' |
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| 22443 | 3' | -53.6 | NC_005056.1 | + | 2011 | 0.71 | 0.320408 |
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Target: 5'- uGAGGACgCC-CUGAAagGUGGCUGCACa -3' miRNA: 3'- uUUUCUGaGGcGGCUU--UACCGGCGUG- -5' |
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| 22443 | 3' | -53.6 | NC_005056.1 | + | 18701 | 1.07 | 0.000805 |
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Target: 5'- cAAAAGACUCCGCCGAAAUGGCCGCACu -3' miRNA: 3'- -UUUUCUGAGGCGGCUUUACCGGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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