Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22443 | 5' | -56.3 | NC_005056.1 | + | 5597 | 0.66 | 0.490311 |
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Target: 5'- uGGGCGCGCauggccgcaAGCgccaccuccUCGGUGAacauGUACg -3' miRNA: 3'- -UCCGCGCGg--------UCG---------AGCCACUgu--UAUGa -5' |
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| 22443 | 5' | -56.3 | NC_005056.1 | + | 3598 | 0.66 | 0.490311 |
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Target: 5'- gAGGCuuGCCGGgucaUCGGUGACGGc--- -3' miRNA: 3'- -UCCGcgCGGUCg---AGCCACUGUUauga -5' |
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| 22443 | 5' | -56.3 | NC_005056.1 | + | 17988 | 0.66 | 0.489241 |
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Target: 5'- aAGGCGCuCCGGCgaagCGGaaacacgaaugagUGACAAUGu- -3' miRNA: 3'- -UCCGCGcGGUCGa---GCC-------------ACUGUUAUga -5' |
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| 22443 | 5' | -56.3 | NC_005056.1 | + | 23802 | 0.67 | 0.418345 |
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Target: 5'- cGGCGCGaucugaCCAGCUugUGGUGACGu---- -3' miRNA: 3'- uCCGCGC------GGUCGA--GCCACUGUuauga -5' |
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| 22443 | 5' | -56.3 | NC_005056.1 | + | 4034 | 0.69 | 0.303292 |
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Target: 5'- aGGGCGUcaccGCCAccGC-CGGUGGCuuUGCUg -3' miRNA: 3'- -UCCGCG----CGGU--CGaGCCACUGuuAUGA- -5' |
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| 22443 | 5' | -56.3 | NC_005056.1 | + | 18736 | 1.08 | 0.000405 |
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Target: 5'- cAGGCGCGCCAGCUCGGUGACAAUACUg -3' miRNA: 3'- -UCCGCGCGGUCGAGCCACUGUUAUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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