Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2247 | 3' | -54.2 | NC_001405.1 | + | 18639 | 0.66 | 0.711011 |
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Target: 5'- gGCuAGGGcggGUUAcaacaacGGCGGACGGCCc- -3' miRNA: 3'- -CGuUCCCa--UAGUa------CCGCCUGCUGGcc -5' |
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| 2247 | 3' | -54.2 | NC_001405.1 | + | 26073 | 0.66 | 0.711011 |
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Target: 5'- cGCAGGcGGUAagcuccgCAuuUGGCGGGCGGgaUUGGu -3' miRNA: 3'- -CGUUC-CCAUa------GU--ACCGCCUGCU--GGCC- -5' |
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| 2247 | 3' | -54.2 | NC_001405.1 | + | 15645 | 0.66 | 0.699973 |
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Target: 5'- -aGAGaGGUAaacUGGCGGAUGAgCUGGg -3' miRNA: 3'- cgUUC-CCAUaguACCGCCUGCU-GGCC- -5' |
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| 2247 | 3' | -54.2 | NC_001405.1 | + | 13955 | 0.67 | 0.632705 |
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Target: 5'- aCGAcGGGUG----GGCGGGCGcggGCCGGg -3' miRNA: 3'- cGUU-CCCAUaguaCCGCCUGC---UGGCC- -5' |
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| 2247 | 3' | -54.2 | NC_001405.1 | + | 33410 | 0.67 | 0.59891 |
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Target: 5'- uGCAAGGcgcuGUAuccaaagcUCAUGGCGGG-GACCa- -3' miRNA: 3'- -CGUUCC----CAU--------AGUACCGCCUgCUGGcc -5' |
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| 2247 | 3' | -54.2 | NC_001405.1 | + | 8237 | 0.69 | 0.489484 |
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Target: 5'- uCGAGGGgaGUUAUGGUGGAUcgGACCa- -3' miRNA: 3'- cGUUCCCa-UAGUACCGCCUG--CUGGcc -5' |
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| 2247 | 3' | -54.2 | NC_001405.1 | + | 9709 | 0.71 | 0.390305 |
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Target: 5'- cGCAA-GGUAggcugagcacCGUGGCGGGCGGCagCGGg -3' miRNA: 3'- -CGUUcCCAUa---------GUACCGCCUGCUG--GCC- -5' |
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| 2247 | 3' | -54.2 | NC_001405.1 | + | 10746 | 0.84 | 0.052958 |
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Target: 5'- cGCGGGcGGUAaccgcauggaUCAcGGCGGACGGCCGGa -3' miRNA: 3'- -CGUUC-CCAU----------AGUaCCGCCUGCUGGCC- -5' |
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| 2247 | 3' | -54.2 | NC_001405.1 | + | 10631 | 1.14 | 0.000403 |
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Target: 5'- cGCAAGGGUAUCAUGGCGGACGACCGGg -3' miRNA: 3'- -CGUUCCCAUAGUACCGCCUGCUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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