miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22470 3' -51.3 NC_005066.1 + 75417 1.09 0.007879
Target:  5'- aGCCGCCCACAAGGACGGCUUAUAAUUa -3'
miRNA:   3'- -CGGCGGGUGUUCCUGCCGAAUAUUAA- -5'
22470 3' -51.3 NC_005066.1 + 159615 0.79 0.485641
Target:  5'- aGCCGaCCACAAGGAuCGGCUUAa---- -3'
miRNA:   3'- -CGGCgGGUGUUCCU-GCCGAAUauuaa -5'
22470 3' -51.3 NC_005066.1 + 97723 0.73 0.775962
Target:  5'- aGCCGUCCcgAAGGACGGCUaAUGu-- -3'
miRNA:   3'- -CGGCGGGugUUCCUGCCGAaUAUuaa -5'
22470 3' -51.3 NC_005066.1 + 97905 0.72 0.856501
Target:  5'- aGCCGUCCuucGGGACGGCUUu----- -3'
miRNA:   3'- -CGGCGGGuguUCCUGCCGAAuauuaa -5'
22470 3' -51.3 NC_005066.1 + 149998 0.72 0.856501
Target:  5'- aGCCGaCCACAuGGAuCGGCUUGUu--- -3'
miRNA:   3'- -CGGCgGGUGUuCCU-GCCGAAUAuuaa -5'
22470 3' -51.3 NC_005066.1 + 75235 0.69 0.95431
Target:  5'- aGCCGUCCuuguGGGCGGCUUu----- -3'
miRNA:   3'- -CGGCGGGuguuCCUGCCGAAuauuaa -5'
22470 3' -51.3 NC_005066.1 + 10969 0.67 0.977165
Target:  5'- aGCCGgaCCuauuCAGGGaAUGGCUUAUGGa- -3'
miRNA:   3'- -CGGCg-GGu---GUUCC-UGCCGAAUAUUaa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.