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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
22472 | 5' | -48.9 | NC_005066.1 | + | 87225 | 0.66 | 0.998753 |
Target: 5'- cGGUGuauauccagaacucGAUgGCGGuUGGACGGCAGa-- -3' miRNA: 3'- -CCAC--------------UUGgUGCCuAUUUGCCGUCaug -5' |
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22472 | 5' | -48.9 | NC_005066.1 | + | 99177 | 0.68 | 0.99447 |
Target: 5'- -uUGcGCCACGGGau--CGGguGUACg -3' miRNA: 3'- ccACuUGGUGCCUauuuGCCguCAUG- -5' |
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22472 | 5' | -48.9 | NC_005066.1 | + | 82306 | 1.13 | 0.009641 |
Target: 5'- cGGUGAACCACGGAUAAACGGCAGUACc -3' miRNA: 3'- -CCACUUGGUGCCUAUUUGCCGUCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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