Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22473 | 5' | -53 | NC_005066.1 | + | 102138 | 0.66 | 0.976842 |
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Target: 5'- uUCCCGuCAcGAGgGgAACCAGUcauACCGa -3' miRNA: 3'- -AGGGC-GUaCUCgUaUUGGUCGu--UGGC- -5' |
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| 22473 | 5' | -53 | NC_005066.1 | + | 158896 | 0.66 | 0.976842 |
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Target: 5'- uUCuuGCGUGAuaaCGUGACCAGauuCCGc -3' miRNA: 3'- -AGggCGUACUc--GUAUUGGUCguuGGC- -5' |
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| 22473 | 5' | -53 | NC_005066.1 | + | 63279 | 0.66 | 0.974264 |
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Target: 5'- ---aGCAUGAGCAUAACCAuGUAAg-- -3' miRNA: 3'- agggCGUACUCGUAUUGGU-CGUUggc -5' |
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| 22473 | 5' | -53 | NC_005066.1 | + | 19004 | 0.66 | 0.971483 |
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Target: 5'- aCCCGC-UGcGGCAUuGCCAaCAAUCGa -3' miRNA: 3'- aGGGCGuAC-UCGUAuUGGUcGUUGGC- -5' |
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| 22473 | 5' | -53 | NC_005066.1 | + | 25027 | 0.66 | 0.968492 |
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Target: 5'- aUCCaCGCGcaauaacauucUGGGUAauAUCAGCAACCu -3' miRNA: 3'- -AGG-GCGU-----------ACUCGUauUGGUCGUUGGc -5' |
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| 22473 | 5' | -53 | NC_005066.1 | + | 148236 | 0.67 | 0.961856 |
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Target: 5'- gUUaaGCAUGaAGC-UAGCgCGGCAACCGc -3' miRNA: 3'- -AGggCGUAC-UCGuAUUG-GUCGUUGGC- -5' |
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| 22473 | 5' | -53 | NC_005066.1 | + | 104129 | 0.67 | 0.950177 |
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Target: 5'- cCCCGCAUucGCAcuUGAUCGGUAcuGCCa -3' miRNA: 3'- aGGGCGUAcuCGU--AUUGGUCGU--UGGc -5' |
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| 22473 | 5' | -53 | NC_005066.1 | + | 135777 | 0.7 | 0.845991 |
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Target: 5'- cCCCGCcacgaauaccgccuuGUGAGCAUGACUuaacGGCAGuCUGa -3' miRNA: 3'- aGGGCG---------------UACUCGUAUUGG----UCGUU-GGC- -5' |
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| 22473 | 5' | -53 | NC_005066.1 | + | 45355 | 0.76 | 0.565793 |
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Target: 5'- aUCCCaGCGUgGAGCAUuACCAGUAACgGc -3' miRNA: 3'- -AGGG-CGUA-CUCGUAuUGGUCGUUGgC- -5' |
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| 22473 | 5' | -53 | NC_005066.1 | + | 82606 | 1.09 | 0.005739 |
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Target: 5'- cUCCCGCAUGAGCAUAACCAGCAACCGc -3' miRNA: 3'- -AGGGCGUACUCGUAUUGGUCGUUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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