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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22475 | 3' | -52.2 | NC_005066.1 | + | 33096 | 0.66 | 0.986388 |
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Target: 5'- aACCAUucACCGAUGAUAcaaugggauugauuCUGGAUCUGCu -3' miRNA: 3'- -UGGUA--UGGCUAUUGUc-------------GGCCUGGAUG- -5' |
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| 22475 | 3' | -52.2 | NC_005066.1 | + | 57947 | 0.67 | 0.977399 |
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Target: 5'- uACCGUAUCaGAUAgacacgcuuuuACAGCCucgcgguccgauGGAUCUACa -3' miRNA: 3'- -UGGUAUGG-CUAU-----------UGUCGG------------CCUGGAUG- -5' |
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| 22475 | 3' | -52.2 | NC_005066.1 | + | 145909 | 0.67 | 0.976906 |
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Target: 5'- cACCAugaccgcuacgcuUACCGAUAAUcucuuuuAGaCCGGACgUGCc -3' miRNA: 3'- -UGGU-------------AUGGCUAUUG-------UC-GGCCUGgAUG- -5' |
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| 22475 | 3' | -52.2 | NC_005066.1 | + | 72904 | 0.67 | 0.969155 |
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Target: 5'- uAUCcgACCGAUucAACGGCUGGugUUGu -3' miRNA: 3'- -UGGuaUGGCUA--UUGUCGGCCugGAUg -5' |
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| 22475 | 3' | -52.2 | NC_005066.1 | + | 93641 | 1.1 | 0.005483 |
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Target: 5'- aACCAUACCGAUAACAGCCGGACCUACg -3' miRNA: 3'- -UGGUAUGGCUAUUGUCGGCCUGGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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