Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22476 | 3' | -40.9 | NC_005066.1 | + | 148447 | 0.66 | 1 |
Target: 5'- ----cGAUgUUACCGAuugcugGAGCGGUuGCc -3' miRNA: 3'- aguaaCUAaAAUGGCU------UUCGCUAuCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 148947 | 0.66 | 1 |
Target: 5'- ---gUGAUuaucuuucUUUGCUGAAAggugauuauaucauuGCGAUGGCa -3' miRNA: 3'- aguaACUA--------AAAUGGCUUU---------------CGCUAUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 156824 | 0.67 | 1 |
Target: 5'- aUCGcUGGgugUACCGGAagaaguauuAGCGAaaUGGCa -3' miRNA: 3'- -AGUaACUaaaAUGGCUU---------UCGCU--AUCG- -5' |
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22476 | 3' | -40.9 | NC_005066.1 | + | 158477 | 0.66 | 1 |
Target: 5'- ----------cACCGAAAGCaGUAGCa -3' miRNA: 3'- aguaacuaaaaUGGCUUUCGcUAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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