miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22477 3' -55.8 NC_005066.1 + 62331 0.66 0.906207
Target:  5'- gCCCUGCaauucaGGCggcGGAUCGUCAgcgAGUg- -3'
miRNA:   3'- gGGGGCGa-----CCGa--CCUAGCAGU---UCAaa -5'
22477 3' -55.8 NC_005066.1 + 87031 0.7 0.702539
Target:  5'- aCCgCaCUGGCUGGuaaCGUCGAGUUUg -3'
miRNA:   3'- gGGgGcGACCGACCua-GCAGUUCAAA- -5'
22477 3' -55.8 NC_005066.1 + 145457 0.66 0.918105
Target:  5'- cCCUCUGCUGGUuuaguUGGcAUagagaaaGUCAAGUUUg -3'
miRNA:   3'- -GGGGGCGACCG-----ACC-UAg------CAGUUCAAA- -5'
22477 3' -55.8 NC_005066.1 + 150251 1.06 0.004593
Target:  5'- uCCCCCGCUGGCUGGAUCGUCAAGUUUc -3'
miRNA:   3'- -GGGGGCGACCGACCUAGCAGUUCAAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.