Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22478 | 5' | -43.7 | NC_005066.1 | + | 113951 | 0.66 | 1 |
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Target: 5'- uUAAAGGGAUUAAacuUGaUGAAUCCUUg-- -3' miRNA: 3'- -AUUUCCCUAGUU---GC-AUUUAGGGAaau -5' |
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| 22478 | 5' | -43.7 | NC_005066.1 | + | 96176 | 0.67 | 0.999999 |
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Target: 5'- uUAAAGGGAUCAccccugaugGCGUAcuuUCUCUc-- -3' miRNA: 3'- -AUUUCCCUAGU---------UGCAUuu-AGGGAaau -5' |
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| 22478 | 5' | -43.7 | NC_005066.1 | + | 33461 | 0.66 | 0.999999 |
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Target: 5'- --cGGGGAUCG-CG-AGAUCCCc--- -3' miRNA: 3'- auuUCCCUAGUuGCaUUUAGGGaaau -5' |
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| 22478 | 5' | -43.7 | NC_005066.1 | + | 10447 | 0.66 | 0.999999 |
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Target: 5'- ---uGGGAUUAGaugAAAUCCCUUUc -3' miRNA: 3'- auuuCCCUAGUUgcaUUUAGGGAAAu -5' |
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| 22478 | 5' | -43.7 | NC_005066.1 | + | 123330 | 0.67 | 0.999996 |
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Target: 5'- -cAGGGGAUC-ACGUAuc-CCCUa-- -3' miRNA: 3'- auUUCCCUAGuUGCAUuuaGGGAaau -5' |
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| 22478 | 5' | -43.7 | NC_005066.1 | + | 92657 | 0.67 | 0.999996 |
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Target: 5'- uUAAAGcGGAUCugUGcGAAUCCCUg-- -3' miRNA: 3'- -AUUUC-CCUAGuuGCaUUUAGGGAaau -5' |
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| 22478 | 5' | -43.7 | NC_005066.1 | + | 62991 | 0.68 | 0.999982 |
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Target: 5'- gAAAGGGAugaUCAAauaAAAUCCCUUUc -3' miRNA: 3'- aUUUCCCU---AGUUgcaUUUAGGGAAAu -5' |
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| 22478 | 5' | -43.7 | NC_005066.1 | + | 158970 | 0.69 | 0.999953 |
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Target: 5'- -cAGGGGAUCGGCG---AUCCCc--- -3' miRNA: 3'- auUUCCCUAGUUGCauuUAGGGaaau -5' |
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| 22478 | 5' | -43.7 | NC_005066.1 | + | 6683 | 0.76 | 0.972501 |
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Target: 5'- -cAAGGGAUCAGCGUAGaccgcguGUUCCUg-- -3' miRNA: 3'- auUUCCCUAGUUGCAUU-------UAGGGAaau -5' |
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| 22478 | 5' | -43.7 | NC_005066.1 | + | 24259 | 0.85 | 0.661684 |
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Target: 5'- aUAAAGGGAUUuACGUuGAUCCCUUUAu -3' miRNA: 3'- -AUUUCCCUAGuUGCAuUUAGGGAAAU- -5' |
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| 22478 | 5' | -43.7 | NC_005066.1 | + | 24068 | 1.07 | 0.059842 |
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Target: 5'- aUAAAGGGAUCAACGUAAAUCCCUUUAu -3' miRNA: 3'- -AUUUCCCUAGUUGCAUUUAGGGAAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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