Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22479 | 5' | -41.7 | NC_005066.1 | + | 48467 | 0.66 | 1 |
Target: 5'- --aGGUUgaccGCUUUAUCGGUGAaugUCUGa -3' miRNA: 3'- guaCUAAaa--UGAAGUAGCUACU---AGGC- -5' |
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22479 | 5' | -41.7 | NC_005066.1 | + | 146007 | 0.66 | 1 |
Target: 5'- --aGGUUgcaaGCgagugUCAUCGAUGAUCgGu -3' miRNA: 3'- guaCUAAaa--UGa----AGUAGCUACUAGgC- -5' |
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22479 | 5' | -41.7 | NC_005066.1 | + | 59971 | 0.66 | 1 |
Target: 5'- uGUGAca-UGCUUCGUUGAUGAUa-- -3' miRNA: 3'- gUACUaaaAUGAAGUAGCUACUAggc -5' |
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22479 | 5' | -41.7 | NC_005066.1 | + | 26503 | 0.67 | 1 |
Target: 5'- aUAUGAaaaUUUUGCUUUua-GGUGAUCUGc -3' miRNA: 3'- -GUACU---AAAAUGAAGuagCUACUAGGC- -5' |
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22479 | 5' | -41.7 | NC_005066.1 | + | 76040 | 0.67 | 1 |
Target: 5'- --cGGUUcgUUAUUUCAUCGGU--UCCGg -3' miRNA: 3'- guaCUAA--AAUGAAGUAGCUAcuAGGC- -5' |
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22479 | 5' | -41.7 | NC_005066.1 | + | 133395 | 0.71 | 0.999987 |
Target: 5'- uCAUGAUagcagggUUAUCGAUGAUCUGg -3' miRNA: 3'- -GUACUAaaauga-AGUAGCUACUAGGC- -5' |
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22479 | 5' | -41.7 | NC_005066.1 | + | 32788 | 1.11 | 0.074341 |
Target: 5'- cCAUGAUUUUACUUCAUCGAUGAUCCGc -3' miRNA: 3'- -GUACUAAAAUGAAGUAGCUACUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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