Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2248 | 3' | -57.6 | NC_001405.1 | + | 11067 | 1.08 | 0.000284 |
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Target: 5'- gCAGCGGCAAGAGCAAGAGCAGCGGCAg -3' miRNA: 3'- -GUCGCCGUUCUCGUUCUCGUCGCCGU- -5' |
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| 2248 | 3' | -57.6 | NC_001405.1 | + | 9728 | 0.69 | 0.25752 |
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Target: 5'- -cGUGGCGGGcGGCAGcGGGUGGCGGUc -3' miRNA: 3'- guCGCCGUUC-UCGUU-CUCGUCGCCGu -5' |
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| 2248 | 3' | -57.6 | NC_001405.1 | + | 9389 | 0.71 | 0.185454 |
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Target: 5'- uGGCGGCGguGGGGgAGGGGggacaCGGCGGCGa -3' miRNA: 3'- gUCGCCGU--UCUCgUUCUC-----GUCGCCGU- -5' |
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| 2248 | 3' | -57.6 | NC_001405.1 | + | 8652 | 0.68 | 0.325498 |
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Target: 5'- -cGCgGGCAGGAGCuGGuGCuGCGcGCGg -3' miRNA: 3'- guCG-CCGUUCUCGuUCuCGuCGC-CGU- -5' |
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| 2248 | 3' | -57.6 | NC_001405.1 | + | 8601 | 0.66 | 0.425431 |
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Target: 5'- gGGgGGCucgggacccgccGGGAGaGGGGGCAGgGGCAc -3' miRNA: 3'- gUCgCCG------------UUCUCgUUCUCGUCgCCGU- -5' |
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| 2248 | 3' | -57.6 | NC_001405.1 | + | 4597 | 0.66 | 0.396398 |
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Target: 5'- --aUGGCAAuGGGCccacGGGCGGCGGCc -3' miRNA: 3'- gucGCCGUU-CUCGuu--CUCGUCGCCGu -5' |
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| 2248 | 3' | -57.6 | NC_001405.1 | + | 3846 | 0.68 | 0.317327 |
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Target: 5'- gCGGUGGCugcAGCGGcugaAGCGGCGGCGg -3' miRNA: 3'- -GUCGCCGuucUCGUUc---UCGUCGCCGU- -5' |
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| 2248 | 3' | -57.6 | NC_001405.1 | + | 2179 | 0.75 | 0.097533 |
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Target: 5'- gCGGCGGCAGGAGC-AGAGCccauggaacccgagAGcCGGCc -3' miRNA: 3'- -GUCGCCGUUCUCGuUCUCG--------------UC-GCCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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