miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22480 5' -46.8 NC_005066.1 + 137657 0.66 0.999924
Target:  5'- uUAUGUGCUaucUGGCGUGGUaUGCguUa -3'
miRNA:   3'- uGUACACGA---AUUGCGCCAaAUGguAg -5'
22480 5' -46.8 NC_005066.1 + 12405 0.66 0.999899
Target:  5'- cCAUGUGUgaugaUUGAUGUGacaUUGCCAUCa -3'
miRNA:   3'- uGUACACG-----AAUUGCGCca-AAUGGUAG- -5'
22480 5' -46.8 NC_005066.1 + 133331 0.66 0.999864
Target:  5'- uGCAUGUacuuucaGCUUugccgAACGUGGUUUACUg-- -3'
miRNA:   3'- -UGUACA-------CGAA-----UUGCGCCAAAUGGuag -5'
22480 5' -46.8 NC_005066.1 + 126756 0.67 0.999778
Target:  5'- cGCGUG-GCgaucaucuCGCGGacagUACCAUCu -3'
miRNA:   3'- -UGUACaCGaauu----GCGCCaa--AUGGUAG- -5'
22480 5' -46.8 NC_005066.1 + 60247 0.67 0.999761
Target:  5'- aACAUGUGCUgcacauACGCuuucaGGUauucauuugauggcUUGCUAUCg -3'
miRNA:   3'- -UGUACACGAau----UGCG-----CCA--------------AAUGGUAG- -5'
22480 5' -46.8 NC_005066.1 + 34492 1.1 0.018343
Target:  5'- uACAUGUGCUUAACGCGGUUUACCAUCu -3'
miRNA:   3'- -UGUACACGAAUUGCGCCAAAUGGUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.