Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22487 | 3' | -44.7 | NC_005068.1 | + | 95804 | 0.66 | 0.999968 |
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Target: 5'- -cAUGAGUCCGAGUUAAuuU-CGuAAAa -3' miRNA: 3'- ucUACUCAGGCUCAAUUuuAcGC-UUU- -5' |
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| 22487 | 3' | -44.7 | NC_005068.1 | + | 56500 | 0.68 | 0.999358 |
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Target: 5'- -uAUGAGUCUGAGUUAAuuUGUa--- -3' miRNA: 3'- ucUACUCAGGCUCAAUUuuACGcuuu -5' |
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| 22487 | 3' | -44.7 | NC_005068.1 | + | 56344 | 0.68 | 0.999196 |
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Target: 5'- -cAUGAGUCUGAGUUAAuuUGUa--- -3' miRNA: 3'- ucUACUCAGGCUCAAUUuuACGcuuu -5' |
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| 22487 | 3' | -44.7 | NC_005068.1 | + | 72045 | 0.78 | 0.835209 |
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Target: 5'- gAGAcGAGUCCGAGUUAAAAU-UGAAAa -3' miRNA: 3'- -UCUaCUCAGGCUCAAUUUUAcGCUUU- -5' |
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| 22487 | 3' | -44.7 | NC_005068.1 | + | 10356 | 0.85 | 0.485064 |
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Target: 5'- -aAUGAGUCCGAGUUAAuuUGUGAAAa -3' miRNA: 3'- ucUACUCAGGCUCAAUUuuACGCUUU- -5' |
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| 22487 | 3' | -44.7 | NC_005068.1 | + | 10155 | 0.91 | 0.276466 |
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Target: 5'- cGAUGAGUCCGAGUUAAAAUcagauuuugGCGAGAa -3' miRNA: 3'- uCUACUCAGGCUCAAUUUUA---------CGCUUU- -5' |
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| 22487 | 3' | -44.7 | NC_005068.1 | + | 3394 | 0.97 | 0.126258 |
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Target: 5'- aAGAUacGAGUCCGAGUUAAAAUGCGAAAa -3' miRNA: 3'- -UCUA--CUCAGGCUCAAUUUUACGCUUU- -5' |
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| 22487 | 3' | -44.7 | NC_005068.1 | + | 47193 | 0.98 | 0.102791 |
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Target: 5'- gAGAcGAGUCCGAGUUAAAAUGCGAAAa -3' miRNA: 3'- -UCUaCUCAGGCUCAAUUUUACGCUUU- -5' |
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| 22487 | 3' | -44.7 | NC_005068.1 | + | 3193 | 1.08 | 0.027921 |
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Target: 5'- gAGAUGAGUCCGAGUUAAAAUGCGAAAa -3' miRNA: 3'- -UCUACUCAGGCUCAAUUUUACGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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