Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22487 | 5' | -45.2 | NC_005068.1 | + | 56460 | 0.66 | 0.999913 |
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Target: 5'- aAAUCgcaaaUUAAgUCGGACUCGUUa -3' miRNA: 3'- -UUAGacuaaAAUUgAGCCUGAGCAGc -5' |
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| 22487 | 5' | -45.2 | NC_005068.1 | + | 56618 | 0.68 | 0.999468 |
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Target: 5'- aAAUCgcaaaUUAACUCaGACUCGUCa -3' miRNA: 3'- -UUAGacuaaAAUUGAGcCUGAGCAGc -5' |
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| 22487 | 5' | -45.2 | NC_005068.1 | + | 10109 | 0.8 | 0.751162 |
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Target: 5'- -----cAUUUUAACUCGGACUCGUCu -3' miRNA: 3'- uuagacUAAAAUUGAGCCUGAGCAGc -5' |
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| 22487 | 5' | -45.2 | NC_005068.1 | + | 3347 | 0.88 | 0.368803 |
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Target: 5'- -cUCUGAUUUUAACUCGGACUCaUCu -3' miRNA: 3'- uuAGACUAAAAUUGAGCCUGAGcAGc -5' |
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| 22487 | 5' | -45.2 | NC_005068.1 | + | 47150 | 0.92 | 0.245294 |
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Target: 5'- aAAUCUGAUUUUAACUCGGACUCaUCu -3' miRNA: 3'- -UUAGACUAAAAUUGAGCCUGAGcAGc -5' |
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| 22487 | 5' | -45.2 | NC_005068.1 | + | 3151 | 1.07 | 0.03126 |
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Target: 5'- gAAUCUGAUUUUAACUCGGACUCGUCGc -3' miRNA: 3'- -UUAGACUAAAAUUGAGCCUGAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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