Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22489 | 3' | -47.3 | NC_005068.1 | + | 10361 | 0.68 | 0.995459 |
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Target: 5'- uGUuuaAUGAGUCCGAGUUAAuuUguGAa -3' miRNA: 3'- gCGc--UGCUCAGGCUCAAUUuuAguCU- -5' |
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| 22489 | 3' | -47.3 | NC_005068.1 | + | 95811 | 0.68 | 0.995459 |
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Target: 5'- aGCcaaccAUGAGUCCGAGUUAAuuUCGu- -3' miRNA: 3'- gCGc----UGCUCAGGCUCAAUUuuAGUcu -5' |
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| 22489 | 3' | -47.3 | NC_005068.1 | + | 3393 | 0.73 | 0.933585 |
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Target: 5'- aGauACGAGUCCGAGUUAAAAUgcGAa -3' miRNA: 3'- gCgcUGCUCAGGCUCAAUUUUAguCU- -5' |
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| 22489 | 3' | -47.3 | NC_005068.1 | + | 3197 | 0.75 | 0.866136 |
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Target: 5'- uGCugaGAUGAGUCCGAGUUAAAAUgcGAa -3' miRNA: 3'- gCG---CUGCUCAGGCUCAAUUUUAguCU- -5' |
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| 22489 | 3' | -47.3 | NC_005068.1 | + | 47192 | 0.79 | 0.690848 |
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Target: 5'- --aGACGAGUCCGAGUUAAAAUgcGAa -3' miRNA: 3'- gcgCUGCUCAGGCUCAAUUUUAguCU- -5' |
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| 22489 | 3' | -47.3 | NC_005068.1 | + | 72044 | 0.81 | 0.581262 |
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Target: 5'- --aGACGAGUCCGAGUUAAAAUUg-- -3' miRNA: 3'- gcgCUGCUCAGGCUCAAUUUUAGucu -5' |
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| 22489 | 3' | -47.3 | NC_005068.1 | + | 10158 | 1.07 | 0.021126 |
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Target: 5'- aCGCGAUGAGUCCGAGUUAAAAUCAGAu -3' miRNA: 3'- -GCGCUGCUCAGGCUCAAUUUUAGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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