Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22492 | 3' | -43.7 | NC_005068.1 | + | 95804 | 0.66 | 0.999994 |
Target: 5'- cAUGAGUCCGAgUUAAUUUcGUa---- -3' miRNA: 3'- cUACUCAGGCUgAAUUAAA-CGcuaaa -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 3034 | 0.67 | 0.999974 |
Target: 5'- aGAUGAGUCgGACUUuGUUUcaaGAUUUc -3' miRNA: 3'- -CUACUCAGgCUGAAuUAAAcg-CUAAA- -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 22932 | 0.68 | 0.99992 |
Target: 5'- uGAUGAGUCUGACUuugaggacuggcuuaUAAag-GCGGUa- -3' miRNA: 3'- -CUACUCAGGCUGA---------------AUUaaaCGCUAaa -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 47016 | 0.68 | 0.999841 |
Target: 5'- uGAUGAGUCgGACUUuGUUU-CGAg-- -3' miRNA: 3'- -CUACUCAGgCUGAAuUAAAcGCUaaa -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 56500 | 0.69 | 0.999432 |
Target: 5'- uAUGAGUCUGAgUUAAUUUGUa---- -3' miRNA: 3'- cUACUCAGGCUgAAUUAAACGcuaaa -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 56344 | 0.7 | 0.999284 |
Target: 5'- cAUGAGUCUGAgUUAAUUUGUa---- -3' miRNA: 3'- cUACUCAGGCUgAAUUAAACGcuaaa -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 10155 | 0.71 | 0.996999 |
Target: 5'- cGAUGAGUCCGAgUUAAaaucagauUUUGgCGAg-- -3' miRNA: 3'- -CUACUCAGGCUgAAUU--------AAAC-GCUaaa -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 47189 | 0.71 | 0.996999 |
Target: 5'- --cGAGUCCGAgUUAAaaUGCGAa-- -3' miRNA: 3'- cuaCUCAGGCUgAAUUaaACGCUaaa -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 3388 | 0.71 | 0.996999 |
Target: 5'- --cGAGUCCGAgUUAAaaUGCGAa-- -3' miRNA: 3'- cuaCUCAGGCUgAAUUaaACGCUaaa -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 56261 | 0.78 | 0.895411 |
Target: 5'- cAUGAGUCCGACUUAAUUUcUGAa-- -3' miRNA: 3'- cUACUCAGGCUGAAUUAAAcGCUaaa -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 3192 | 0.8 | 0.827054 |
Target: 5'- aGAUGAGUCCGAgUUAAaaUGCGAa-- -3' miRNA: 3'- -CUACUCAGGCUgAAUUaaACGCUaaa -5' |
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22492 | 3' | -43.7 | NC_005068.1 | + | 10356 | 0.83 | 0.653563 |
Target: 5'- aAUGAGUCCGAgUUAAUUUGUGAa-- -3' miRNA: 3'- cUACUCAGGCUgAAUUAAACGCUaaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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