Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22492 | 5' | -37.1 | NC_005068.1 | + | 56231 | 0.74 | 1 |
Target: 5'- -aAAAUUUuaaaaaUCGcAAAUUAAGUCGGACu -3' miRNA: 3'- aaUUUAAA------AGUcUUUAAUUCAGCCUG- -5' |
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22492 | 5' | -37.1 | NC_005068.1 | + | 56471 | 0.73 | 1 |
Target: 5'- -aAAAUUUuuaaaaaUCGcAAAUUAAGUCGGACu -3' miRNA: 3'- aaUUUAAA-------AGUcUUUAAUUCAGCCUG- -5' |
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22492 | 5' | -37.1 | NC_005068.1 | + | 56315 | 0.73 | 1 |
Target: 5'- -aAAAUUUuuaaaaaUCGcAAAUUAAGUCGGACu -3' miRNA: 3'- aaUUUAAA-------AGUcUUUAAUUCAGCCUG- -5' |
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22492 | 5' | -37.1 | NC_005068.1 | + | 3040 | 0.7 | 1 |
Target: 5'- ------aUUguGAGA-UGAGUCGGACu -3' miRNA: 3'- aauuuaaAAguCUUUaAUUCAGCCUG- -5' |
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22492 | 5' | -37.1 | NC_005068.1 | + | 47028 | 0.69 | 1 |
Target: 5'- -cGAAUUcUguGAugaUGAGUCGGACu -3' miRNA: 3'- aaUUUAAaAguCUuuaAUUCAGCCUG- -5' |
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22492 | 5' | -37.1 | NC_005068.1 | + | 73621 | 0.69 | 1 |
Target: 5'- -aGGAUUUUCAGGAcgUAcuGGUUGGu- -3' miRNA: 3'- aaUUUAAAAGUCUUuaAU--UCAGCCug -5' |
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22492 | 5' | -37.1 | NC_005068.1 | + | 67369 | 0.67 | 1 |
Target: 5'- --------cCAGAAAaacaauucuauuuUUGAGUCGGGCc -3' miRNA: 3'- aauuuaaaaGUCUUU-------------AAUUCAGCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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