Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
225 | 5' | -62.9 | AC_000008.1 | + | 33228 | 0.66 | 0.269588 |
Target: 5'- aCUGCUGCCG-CCGCCGCuccGUcCUgCAGg -3' miRNA: 3'- -GGCGACGGCgGGCGGUGc--CAcGA-GUC- -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 16198 | 0.66 | 0.269588 |
Target: 5'- cCCGgcaCUGCCGCCCaacgcgcgGCgGCGGcccUGCUUAa -3' miRNA: 3'- -GGC---GACGGCGGG--------CGgUGCC---ACGAGUc -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 18142 | 0.66 | 0.25004 |
Target: 5'- aCGCaUGCCGCCCGUCA-GGccgugGC-CGGc -3' miRNA: 3'- gGCG-ACGGCGGGCGGUgCCa----CGaGUC- -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 23805 | 0.66 | 0.24815 |
Target: 5'- gCCGCUGCCcguGCCaGCCAgGGcccuuugcaggcugUGCaUCAGc -3' miRNA: 3'- -GGCGACGG---CGGgCGGUgCC--------------ACG-AGUC- -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 27350 | 0.67 | 0.225812 |
Target: 5'- cCCGCggcccaCCGCCCGCCgcGCGGUaccguagucGCgccgCGGg -3' miRNA: 3'- -GGCGac----GGCGGGCGG--UGCCA---------CGa---GUC- -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 3656 | 0.68 | 0.188215 |
Target: 5'- gCGCaUGCC-CCCauggGCCGgGGUGCgUCAGa -3' miRNA: 3'- gGCG-ACGGcGGG----CGGUgCCACG-AGUC- -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 20832 | 0.68 | 0.183316 |
Target: 5'- uUGCUGCCGCCugCGCCACccccgcccUGUUCGGu -3' miRNA: 3'- gGCGACGGCGG--GCGGUGcc------ACGAGUC- -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 5355 | 0.69 | 0.152092 |
Target: 5'- -gGCUGCgCGCUgGCCAgGGUGCg--- -3' miRNA: 3'- ggCGACG-GCGGgCGGUgCCACGaguc -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 29244 | 0.7 | 0.129256 |
Target: 5'- gCGCuUGCCGCCCGCgCAUGGcag-CAGg -3' miRNA: 3'- gGCG-ACGGCGGGCG-GUGCCacgaGUC- -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 19628 | 0.75 | 0.052797 |
Target: 5'- cCCGCaugGCCGCCCGUCGgcCGGUGCg--- -3' miRNA: 3'- -GGCGa--CGGCGGGCGGU--GCCACGaguc -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 9187 | 0.75 | 0.049871 |
Target: 5'- gCCGCUGCCG-CUGCCGcCGGUGCa--- -3' miRNA: 3'- -GGCGACGGCgGGCGGU-GCCACGaguc -5' |
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225 | 5' | -62.9 | AC_000008.1 | + | 26148 | 1.09 | 0.000116 |
Target: 5'- cCCGCUGCCGCCCGCCACGGUGCUCAGc -3' miRNA: 3'- -GGCGACGGCGGGCGGUGCCACGAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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