Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2250 | 5' | -50.6 | NC_001405.1 | + | 16213 | 0.67 | 0.799252 |
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Target: 5'- --cGCGGCGGCGGCccugcuuaaccgcgcACGUcGCAccGGCCg -3' miRNA: 3'- caaUGUUGUCGCCGu--------------UGCA-CGU--CUGG- -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 34856 | 0.66 | 0.808466 |
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Target: 5'- --gGCGGggaAGUGGCcgcgcuggucCGUGCAGACCu -3' miRNA: 3'- caaUGUUg--UCGCCGuu--------GCACGUCUGG- -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 28767 | 0.66 | 0.828311 |
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Target: 5'- -gUGCAGCAgGCGGCAACccuaAGGCa -3' miRNA: 3'- caAUGUUGU-CGCCGUUGcacgUCUGg -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 9731 | 0.66 | 0.828311 |
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Target: 5'- --gGCG--GGCGGCAGCG-GguGGCg -3' miRNA: 3'- caaUGUugUCGCCGUUGCaCguCUGg -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 15362 | 0.66 | 0.837885 |
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Target: 5'- -aUACAACuaCGGCGACccuCAGACCg -3' miRNA: 3'- caAUGUUGucGCCGUUGcacGUCUGG- -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 9472 | 0.66 | 0.84721 |
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Target: 5'- --gGCGACGGCgcauggucucGGUGACG-GCGcGGCCg -3' miRNA: 3'- caaUGUUGUCG----------CCGUUGCaCGU-CUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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