Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2250 | 5' | -50.6 | NC_001405.1 | + | 26741 | 0.8 | 0.170574 |
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Target: 5'- --gGCGGCAGCGGCAGCG-GCAG-Ca -3' miRNA: 3'- caaUGUUGUCGCCGUUGCaCGUCuGg -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 26828 | 0.74 | 0.396496 |
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Target: 5'- --cACAGCGGCGGCAGCa-GCAGGa- -3' miRNA: 3'- caaUGUUGUCGCCGUUGcaCGUCUgg -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 28767 | 0.66 | 0.828311 |
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Target: 5'- -gUGCAGCAgGCGGCAACccuaAGGCa -3' miRNA: 3'- caAUGUUGU-CGCCGUUGcacgUCUGg -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 33280 | 0.74 | 0.39552 |
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Target: 5'- --aGCGGCGGCGGCAgcaguuuauucgcGCGcugcUGCAGcACCa -3' miRNA: 3'- caaUGUUGUCGCCGU-------------UGC----ACGUC-UGG- -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 34856 | 0.66 | 0.808466 |
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Target: 5'- --gGCGGggaAGUGGCcgcgcuggucCGUGCAGACCu -3' miRNA: 3'- caaUGUUg--UCGCCGuu--------GCACGUCUGG- -5' |
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| 2250 | 5' | -50.6 | NC_001405.1 | + | 34904 | 0.72 | 0.501301 |
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Target: 5'- uGUUGCAugGGCGGCGAUauaaaaUGCAGguGCUg -3' miRNA: 3'- -CAAUGUugUCGCCGUUGc-----ACGUC--UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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